Articles
Silver nitrate (AgNO3) can suppress expression levels of WOUND INDUCED DEDIFFERENTIATION1 (WIND1) gene via direct organogenesis of Euphorbia pulcherrima Christmas Eve
Article number
1417_5
Pages
37 – 42
Language
English
Abstract
Poinsettia (Euphorbia pulcherrima) belongs to the Euphorbiaceae family which is recognized as one of the best-known perennial flowering plants associated with the winter season in many parts of the world.
Due to the low propagation coefficient of traditional propagation methods and the disadvantage of transferring various diseases and pathogens including bacterial and fungal contaminations, the use of plant tissue culture techniques has been sought with their many advantages such as season-free, mass-scale, and healthy production in a limited period.
Moreover, successful genetic engineering programs require establishing several biotechnological steps particularly direct/indirect organogenesis and somatic embryogenesis procedures.
Therefore, in this study, two different in vitro culture media including Murashige and Skoog (MS) and Driver and Kuniyuki (DKW) in combination with various concentrations of silver nitrate (AgNO3) (0, 5, 10, 20 mg L‑1) were tested for their viability in direct organogenesis of Euphorbia pulcherrima Christmas Eve via stem segments.
A stable concentration of 1 mg L‑1 BAP + 0.1 mg L‑1 NAA was applied for all treatments.
Our results indicated that the highest concentration of silver nitrate (20 mg L‑1) could control internal bacterial infections and enhance the direct regeneration rate in comparison with the control group.
Upregulation of shoot regulatory genes including WUSCHEL (WUS) and suppression of WOUND INDUCED DEDIFFERENTIATION (WIND1) confirmed the positive effect of silver nitrate on increasing regeneration rate.
Due to the low propagation coefficient of traditional propagation methods and the disadvantage of transferring various diseases and pathogens including bacterial and fungal contaminations, the use of plant tissue culture techniques has been sought with their many advantages such as season-free, mass-scale, and healthy production in a limited period.
Moreover, successful genetic engineering programs require establishing several biotechnological steps particularly direct/indirect organogenesis and somatic embryogenesis procedures.
Therefore, in this study, two different in vitro culture media including Murashige and Skoog (MS) and Driver and Kuniyuki (DKW) in combination with various concentrations of silver nitrate (AgNO3) (0, 5, 10, 20 mg L‑1) were tested for their viability in direct organogenesis of Euphorbia pulcherrima Christmas Eve via stem segments.
A stable concentration of 1 mg L‑1 BAP + 0.1 mg L‑1 NAA was applied for all treatments.
Our results indicated that the highest concentration of silver nitrate (20 mg L‑1) could control internal bacterial infections and enhance the direct regeneration rate in comparison with the control group.
Upregulation of shoot regulatory genes including WUSCHEL (WUS) and suppression of WOUND INDUCED DEDIFFERENTIATION (WIND1) confirmed the positive effect of silver nitrate on increasing regeneration rate.
Authors
M.D. Pahnekolayi, V.G. Schmidt, T. Sprink, R. Boehm, H. Halbwirth
Keywords
direct regeneration, in vitro culture, ornamental plants, regeneration genes, silver compounds
Groups involved
- Division Vine and Berry Fruits
- Division Plant-Environment Interactions in Field Systems
- Division Horticulture for Human Health
- Working Group Vaccinium Species and Management
- Working Group Rubus and Ribes Species and Management
- Working Group Kiwifruit Culture and Management
- Working Group Strawberry Culture and Management
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