Articles
In vitro propagation of Limbarda crithmoides (L.) Dumort. and the effect of NaCl
Article number
1417_28
Pages
221 – 228
Language
English
Abstract
The present study aimed to develop an in vitro establishment and multiplication protocol for Limbarda crithmoides (L.) Dumort., a species that can be used in floriculture and landscape architecture.
Nodal explants were cultured on solid (8 g L‑1 agar) Murashige and Skoog (MS) or Rugini Olive (OM) medium, supplemented with 0.5 mg L‑1 6-benzyladenine (BA) or with no growth regulators (Hf). After the in vitro establishment, two subsequent subcultures took place on the same media with 0.5 mg L‑1 BA or Kinetin (KIN) for the first and second subcultures.
Media (MS and OM) with no growth regulators were used as controls.
The multiplication phase was on OM supplemented with 0.2 or 0.5 mg L‑1 BA, with or without 0.01 mg L‑1 1-naphthaleneacetic acid (NAA). The in vitro response when shoots were cultured on OM media supplemented with several NaCl concentrations (5, 15, 25, or 50 g L‑1) was evaluated too.
The results showed that a successful establishment (80-95%) was achieved in all the tested media; a pronounced shoot length was recorded when OM media were used, while an enhanced number of nodes explant‑1 was observed when explants were cultured on MS with 0.5 mg L‑1 BA. After the subsequent two subcultures, OM was confirmed to induce a more pronounced shoot length.
The number of short shoots increased during the subculture on MS or OM media supplemented with 0.5 mg L‑1 BA. The addition of 0.5 mg L‑1 KIN to MS and OM media, during the second subculture, did not promote the proliferation rate.
The best multiplication index (MI) was achieved on OM either Hf or in the presence of BA at 0.2 mg L‑1, with or without NAA at 0.01 mg L‑1. When NaCl was added to the OM medium, shoot formation was 100% for all the NaCl concentrations tested, except 50 g L‑1 (50%), and only LSQUOshortRSQUO shoots were formed on this medium.
No differences were observed between OM Hf media and media containing 5 g L‑1 NaCl.
Spontaneous rooting occurred on all cultures reaching 100% on OM Hf media and media containing 5-15 g L‑1 NaCl.
Root formation on OM with 25 g L‑1 NaCl was only 10%, while no root formation occurred on OM with 50 g L‑1 NaCl.
Our results can be useful to set an in vitro propagation of L. crithmoides which can valorise this species.
Nodal explants were cultured on solid (8 g L‑1 agar) Murashige and Skoog (MS) or Rugini Olive (OM) medium, supplemented with 0.5 mg L‑1 6-benzyladenine (BA) or with no growth regulators (Hf). After the in vitro establishment, two subsequent subcultures took place on the same media with 0.5 mg L‑1 BA or Kinetin (KIN) for the first and second subcultures.
Media (MS and OM) with no growth regulators were used as controls.
The multiplication phase was on OM supplemented with 0.2 or 0.5 mg L‑1 BA, with or without 0.01 mg L‑1 1-naphthaleneacetic acid (NAA). The in vitro response when shoots were cultured on OM media supplemented with several NaCl concentrations (5, 15, 25, or 50 g L‑1) was evaluated too.
The results showed that a successful establishment (80-95%) was achieved in all the tested media; a pronounced shoot length was recorded when OM media were used, while an enhanced number of nodes explant‑1 was observed when explants were cultured on MS with 0.5 mg L‑1 BA. After the subsequent two subcultures, OM was confirmed to induce a more pronounced shoot length.
The number of short shoots increased during the subculture on MS or OM media supplemented with 0.5 mg L‑1 BA. The addition of 0.5 mg L‑1 KIN to MS and OM media, during the second subculture, did not promote the proliferation rate.
The best multiplication index (MI) was achieved on OM either Hf or in the presence of BA at 0.2 mg L‑1, with or without NAA at 0.01 mg L‑1. When NaCl was added to the OM medium, shoot formation was 100% for all the NaCl concentrations tested, except 50 g L‑1 (50%), and only LSQUOshortRSQUO shoots were formed on this medium.
No differences were observed between OM Hf media and media containing 5 g L‑1 NaCl.
Spontaneous rooting occurred on all cultures reaching 100% on OM Hf media and media containing 5-15 g L‑1 NaCl.
Root formation on OM with 25 g L‑1 NaCl was only 10%, while no root formation occurred on OM with 50 g L‑1 NaCl.
Our results can be useful to set an in vitro propagation of L. crithmoides which can valorise this species.
Authors
A. Paraskevopoulou, K. Bertsouklis, K.-E. Meleggoglou
Keywords
halophytes, micropropagation, ornamentals, root formation, salinity
Groups involved
- Division Vine and Berry Fruits
- Division Plant-Environment Interactions in Field Systems
- Division Horticulture for Human Health
- Working Group Vaccinium Species and Management
- Working Group Rubus and Ribes Species and Management
- Working Group Kiwifruit Culture and Management
- Working Group Strawberry Culture and Management
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