Articles
In vitro response of Kosteletzkya pentacarpos on media containing acetylsalicylic or salicylic acid
Article number
1417_26
Pages
207 – 214
Language
English
Abstract
In the present study, the effect of acetylsalicylic (ASA) and salicylic (SA) acid on in vitro propagation of Kosteletzkya pentacarpos was studied.
Nodal explants were excised from in vitro grown plantlets and cultured on solid (8 g L‑1 agar), Murashige and Skoog (MS) medium, containing 30 g L‑1 sucrose supplemented with ASA or SA at 0.01, 0.05, 0.1, and 0.5 mg L‑1. To assess the in vitro response, analyses of plant pigments, i.e., chlorophyll (Chl) a, b, total (a+b), and carotenoids were performed.
For the extraction of the pigments, 0.5 g of in vitro-grown micro-shoots were homogenized in 80% acetone in the dark and analysed spectrophotometrically.
The percentage of shoot regeneration was high (92-100%) on both the control medium (MS medium + 30 g L‑1) and media supplemented with 0.01, 0.05, and 0.1 mg L‑1 ASA or SA. When ASA or SA were supplemented at 0.5 mg L‑1, the percentage of shoot regeneration drastically dropped.
For all the treatments, only one shoot per explant was recorded.
The growth parameters (shoot length and number of nodes per explant) were satisfactory and similar for shoots grown on a control medium and media supplemented with ASA or SA 0.01 mg L‑1. The best multiplication index (MI=39) was recorded when shoots were grown on MS supplemented with ASA 0.01 mg L‑1. Shoots spontaneously rooted in the tested media and the rooting percentage was about 90% when shoots were cultured on a control medium or media containing 0.01 mg L‑1 ASA and SA. The rooting efficiency decreased gradually with increasing concentrations of ASA and SA. The Chl a, Chl total, and carotenoid content was in agreement with the in vitro performance of shoots, and the lowest content was recorded for shoots grown on media with ASA and SA at 0.05 mg L‑1.
Nodal explants were excised from in vitro grown plantlets and cultured on solid (8 g L‑1 agar), Murashige and Skoog (MS) medium, containing 30 g L‑1 sucrose supplemented with ASA or SA at 0.01, 0.05, 0.1, and 0.5 mg L‑1. To assess the in vitro response, analyses of plant pigments, i.e., chlorophyll (Chl) a, b, total (a+b), and carotenoids were performed.
For the extraction of the pigments, 0.5 g of in vitro-grown micro-shoots were homogenized in 80% acetone in the dark and analysed spectrophotometrically.
The percentage of shoot regeneration was high (92-100%) on both the control medium (MS medium + 30 g L‑1) and media supplemented with 0.01, 0.05, and 0.1 mg L‑1 ASA or SA. When ASA or SA were supplemented at 0.5 mg L‑1, the percentage of shoot regeneration drastically dropped.
For all the treatments, only one shoot per explant was recorded.
The growth parameters (shoot length and number of nodes per explant) were satisfactory and similar for shoots grown on a control medium and media supplemented with ASA or SA 0.01 mg L‑1. The best multiplication index (MI=39) was recorded when shoots were grown on MS supplemented with ASA 0.01 mg L‑1. Shoots spontaneously rooted in the tested media and the rooting percentage was about 90% when shoots were cultured on a control medium or media containing 0.01 mg L‑1 ASA and SA. The rooting efficiency decreased gradually with increasing concentrations of ASA and SA. The Chl a, Chl total, and carotenoid content was in agreement with the in vitro performance of shoots, and the lowest content was recorded for shoots grown on media with ASA and SA at 0.05 mg L‑1.
Authors
K. Bertsouklis, K.-S. Arfani, N. Ntoulas, S. Hatzilazarou, S. Kostas, M. Villani
Keywords
chlorophyll content, micropropagation, plant tissue culture, rooting, salt-tolerant plant
Groups involved
- Division Vine and Berry Fruits
- Division Plant-Environment Interactions in Field Systems
- Division Horticulture for Human Health
- Working Group Vaccinium Species and Management
- Working Group Rubus and Ribes Species and Management
- Working Group Kiwifruit Culture and Management
- Working Group Strawberry Culture and Management
Online Articles (35)