Articles
Toward the identification of quantitative resistance mechanisms of rose to black spot disease
Article number
1417_4
Pages
29 – 36
Language
English
Abstract
Black spot disease, caused by the hemibiotrophic fungus Diplocarpon rosae, is one of the main foliar diseases affecting garden roses.
Resistant cultivars have proven to be an efficient alternative to the use of fungicides, but the genetic basis of disease resistance is not well known.
Yet, understanding the underlying mechanisms of resistance is essential for an effective and sustainable deployment of resistant cultivars.
The RW genotype, a hybrid of Rosa wichurana, exhibits quantitative resistance to rose black spot.
Analysis of an F1 progeny from a cross between the susceptible cultivar Rosa chinensis ‘Old Blush’ and RW showed that the resistance of RW results mainly from the combined action of two QTLs, located on linkage groups 3 and 5. The objective of this study was to better understand the molecular mechanisms underlying these QTLs.
Thus, RNA sequencing was carried out on inoculated and non-inoculated samples of RW plants, at three different stages of the RW-D. rosae interaction.
The comparison of inoculated and non-inoculated samples at each time point has revealed interesting shifts in the expression of genes associated with plant defense, but also signaling, phytohormones, and cell wall organization.
We hypothesize that the quantitative nature of this resistance may result in this complex pattern of gene expression and propose some perspectives to go further in the study of rose black spot resistance.
Resistant cultivars have proven to be an efficient alternative to the use of fungicides, but the genetic basis of disease resistance is not well known.
Yet, understanding the underlying mechanisms of resistance is essential for an effective and sustainable deployment of resistant cultivars.
The RW genotype, a hybrid of Rosa wichurana, exhibits quantitative resistance to rose black spot.
Analysis of an F1 progeny from a cross between the susceptible cultivar Rosa chinensis ‘Old Blush’ and RW showed that the resistance of RW results mainly from the combined action of two QTLs, located on linkage groups 3 and 5. The objective of this study was to better understand the molecular mechanisms underlying these QTLs.
Thus, RNA sequencing was carried out on inoculated and non-inoculated samples of RW plants, at three different stages of the RW-D. rosae interaction.
The comparison of inoculated and non-inoculated samples at each time point has revealed interesting shifts in the expression of genes associated with plant defense, but also signaling, phytohormones, and cell wall organization.
We hypothesize that the quantitative nature of this resistance may result in this complex pattern of gene expression and propose some perspectives to go further in the study of rose black spot resistance.
Authors
L. Lambelin, T. Thouroude, J. Jeauffre, J. Chameau, C. Vilfroy, C. Boursier, S. Aubourg, S. Pelletier, D.C. Lopez Arias, L. Hibrand-Saint Oyant, V. Soufflet-Freslon, F. Foucher, S. Paillard
Keywords
defense response, Diplocarpon rosae, resistance QTL, RNA sequencing, Rosa
Groups involved
- Division Vine and Berry Fruits
- Division Plant-Environment Interactions in Field Systems
- Division Horticulture for Human Health
- Working Group Vaccinium Species and Management
- Working Group Rubus and Ribes Species and Management
- Working Group Kiwifruit Culture and Management
- Working Group Strawberry Culture and Management
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