Home > I International Jujube Symposium > AN IMPROVED PROTOCOL SUITABLE FOR POLYMORPHISM STUDIES IN CHINESE JUJUBE (ZIZIPHUS JUJUBA MILL.)

Articles

AN IMPROVED PROTOCOL SUITABLE FOR POLYMORPHISM STUDIES IN CHINESE JUJUBE (ZIZIPHUS JUJUBA MILL.)

Article number

840_11

Pages

97 – 106

Language

English

Abstract

An efficient and stable AFLP protocol for Chinese jujube was established and validated with several cultivars.
Major steps influencing in AFLP procedure were the extraction and purification of genomic DNA, restriction and ligation of adapter into the restricted genomic DNA, pre-amplification reaction, selective amplification reaction, electrophoresis and sliver-staining.
The genomic DNA extracted by standardized modified CTAB method was suitable for downstream process in AFLP procedure.
Four hundred ng DNA was digested with 3 units of EcoRI and MseI enzymes respectively and incubated at 37°C for 5 h, the digested DNA fragment was diluted to 5 times and used as templates for pre-amplification, and the pre-amplification products were diluted to 10 times and used as templates for selective amplification, which was found suitable for Chinese jujube in AFLP. The electrophoresis and silver-staining were done as per normal procedure.
This standardized method had also been validated with seven cultivars belonging to Chinese jujube.

Publication

I International Jujube Symposium

Authors

R.X. Bai, J.Y. Peng, L. Li, Y. Zhang, B. Han, L.S. Zhang, X.Y. Li

Keywords

Chinese jujube, AFLP protocol, polymorphism, relationship, cultivars

Full text

https://doi.org/10.17660/ActaHortic.2009.840.11

Online Articles (80)

1. GERMPLASM RESOURCES AND PRODUCTION OF JUJUBE IN CHINA

M.J. Liu | Z.H. Zhao

2. GENETIC DIVERSITY AND STATUS OF ZIZIPHUS IN INDIA

O.P. Awasthi | T.A. More

3. SELECTED ISSUES IN MODERN CULTIVATION OF FRUIT TREES RELEVANT TO JUJUBE

A. Blumenfeld

4. INTRODUCTION, EVALUATION AND PROPAGATION OF ZIZIPHUS IN KUWAIT

C. Sudhersan | J.H. Ashkanani

5. EVALUATION OF VARIETIES AND STANDARDIZATION OF PRODUCTION TECHNOLOGIES IN BER (ZIZIPHUS MAURITIANA) UNDER RAINFED VERTISOLS

S. Anbu | S. Balasubramanyan | K. Venkatesan | M. Selvarajan | R. Duarisingh

6. THE CURRENT DEVELOPMENT AND PROMISE OF FRESH JUJUBE INDUSTRY IN THE SOUTH OF CHINA

B.X. Xie | Z.Y. Gu | S. Wang

7. STATISTICAL ANALYSIS OF INTERNATIONAL RESEARCH LITERATURE ON THE GENUS ZIZIPHUS MILL.

Y.F. Qi | L. Dai | M.J. Liu

8. AQUIFER: A COORDINATE EFFORT IN LINE WITH REGENERATION OF THE KONAR STANDS (ZIZIPHUS SPINA-CHRISTI (L.) WILD.) IN SOUTHERN IRAN

S.M. Sadeghi | A. Jafary | N. Farrar

9. INTERNATIONAL SURVEY AND DISCUSSION ON THE NAMES OF GENUS JUJUBE AND ITS MAIN SPECIES

M.J. Liu | Z.H. Zhao | Y.F. Qi

10. INFRASPECIFIC CLASSIFICATION OF ZIZIPHUS JUJUBA MILL. USING AFLP MARKER TECHNIQUE

R.X. Bai | J.Y. Peng | L. Li | Y. Zhang | X.Y. Li

11. AN IMPROVED PROTOCOL SUITABLE FOR POLYMORPHISM STUDIES IN CHINESE JUJUBE (ZIZIPHUS JUJUBA MILL.)

12. THE PHYLOGENETIC RELATIONSHIPS OF GENUS ZIZIPHUS MILL. NATIVE TO CHINA BASED ON RAPD ANALYSIS

L. Li | J.Y. Peng | R.X. Bai | R.H. Huang | B.Q. Zheng

13. AFLP ANALYSIS OF GENETIC RELATIONSHIP AND DISCRIMINATION OF CHINESE JUJUBE GERMPLASM RESOURCES

Y.F. Wen | Q.Q. Yuan | S. Wang | G. He

14. ANALYSIS OF QTLS FOR FOUR AGRONOMIC CHARACTERS IN CHINESE JUJUBE

15. GENETIC ANALYSIS OF ZIZIPHUS JUJUBA ‘HUIZAO’ USING ISSR MARKERS

J.D. Li | H.T. Bi | H.T. Li | Z.S. Li | J.C. Feng

16. THE ORTHOGONAL OPTIMIZATION OF SRAP AMPLIFICATION SYSTEM IN CHINESE JUJUBE

L. Li | J.Y. Peng | R.H. Huang | R.X. Bai | B.Q. Zheng

17. GENETIC VARIATIONS OF ZIZIPHUS CULTIVAR ‘ZANHUANGDAZAO’ BY USING RAPD TECHNIQUE

18. GENETIC VARIATION OF SOUR JUJUBE IN TAIHANG MOUNTAIN BY USING RAPD TECHNIQUE

19. SALT TOLERANCE OF FOUR JUJUBE CULTIVARS

20. STUDY ON THIRTEEN AGRONOMIC TRAITS OF ‘DONGZAO’ × ‘LINYILIZAO’ HYBRID PROGENY

Y.H. Li | L.Y. Shen | Y.M. Mao | J. Liu

21. COMPARATIVE STUDIES ON JUJUBE CULTIVAR ‘ZHONGQUIUSUCUIZAO’ WITH SIX DIFFERENT JUJUBE CULTIVARS

Z.Y. Gu | B.X. Xie | S. Wang

22. PERFORMANCE OF BER CULTIVARS RAISED THROUGH TOP-WORKING IN CLOSE SPACING

S.N. Ghosh | R.K. Tarai | B. Bera | A. Kundu

23. ECOLOGICAL INVESTIGATION ON CHRIST-THORN (ZIZIPHUS SPINA-CHRISTI L.) IN BUSHEHR PROVINCE

S.M. Sadeghi | K. Javanshir | M. Namiranian | H. Lotfian

24. VARIATION IN MORPHOLOGY OF JUJUBE ‘MUZAO’ (ZIZIPHUS JUJUBA MILL.) IN THE LOSSES PLATEAU OF CHINA

W.H. Gao | X.G. Li | C.Z. Wang

25. AGRONOMIC DIVERSITY OF SOUR JUJUBE (ZIZIPHUS ACIDOJUJUBA C.Y. CHENG ET M.J. LIU) IN CHINA

26. INVESTIGATION ON THE CHARACTERISTICS OF FRUITING AND SEED DEVELOPMENT IN CHINESE JUJUBE (ZIZIPHUS JUJUBA MILL.)

27. THE DISTRIBUTION AND GRADING OF BIOLOGICAL CHARACTERS IN ZIZIPHUS JUJUBA MILL.

Y.H. Dong | P. Liu | M.J. Liu | J.Y. Peng | D.K. Li

28. CORE COLLECTION CONSTRUCTION BASED ON BOTANIC AND AGRONOMIC CHARACTERS IN CHINESE JUJUBE

29. CONSERVATION, CHARACTERIZATION, EVALUATION AND UTILIZATION OF CHINESE JUJUBE GERMPLASM RESOURCES

Y.K. Wang | D.K. Li | C.L. Sui | A.L. Zhao | X.M. Du

30. ‘MIGUANXIN 1’, A NEW CRISP TABLE CULTIVAR OF JUJUBE (ZIZIPHUS JUJUBA MILL.)

C.Z. Wang | W.H. Gao | X.G. Li

31. ‘YANLIANGCUIZAO’, A NEW MIDDLE-EARLY MATURATING CULTIVAR OF JUJUBE (ZIZIPHUS JUJUBA MILL.) FOR FRESH CONSUMING

C.Z. Wang | X.G. Li | W. Meng

32. A NEW FRESH USED JUJUBE CULTIVAR ‘ZHONGQIUSUCUI’ FOR THE SOUTH OF CHINA

33. TWO NEW WILD JUJUBE CULTIVARS – ‘DASUANZAO’ AND ‘CUISUANZAO’

D.Z. Liu | C.T. Wang | T.J. Yu | L.Q. Hou | D.C. Zhao

34. CHARACTERIZATION OF TWO ROMANIAN LOCAL BIOTYPES OF ZIZIPHUS JUJUBE

F. Stănică

35. PLANT REGENERATION FROM IMMATURE EMBRYO IN CHINESE JUJUBE (ZIZIPHUS JUJUBA MILL.)

J.R. Wang | M. Zhang | M.J. Liu*

36. DEVELOPMENT OF SOMATIC EMBRYO AND ADVENTITIOUS BUDS FROM THE DIFFERENTIATING CULTURED CALLI OF CHINESE JUJUBE MU-ZAO

37. KEY FACTOR OF TISSUE CULTURE AND PRELIMINARY OBSERVATION OF GROWTH AND BEARING OF CHINESE JUJUBE JINCHANG NO.1

Q. Cao | J.J. Du

38. DIRECT SHOOT REGENERATION FROM LEAF EXPLANTS OF SOUR JUJUBE

N. Wang | M.J. Liu | Z.H. Zhao | Z.Y. Qin

39. TISSUE CULTURE OF CHINESE JUJUBE USING DIFFERENT EXPLANTS

L. Dai | J. Zhao | M.J. Liu

40. HIGH EFFICIENCY SHOOT REGENERATION FROM LEAF EXPLANTS OF TAISHAN SOUR JUJUBE (ZIZYPHUS SPINOSUS HU.)

H. Sun | Q. Sun | G. Zhou | Q. Liu | L. Xin

41. EFFECT OF AUXINS ON IN VITRO PROPAGATION OF SOUR JUJUBE INFECTED BY WITCHES’ BROOM PHYTOPLASMA

Q. Sun | G. Zhou | H. Sun | G. Giang

42. EFFECT OF PLANT GROWTH REGULATORS ON TISSUE CULTURE IN CHINESE JUJUBE

R.J. Zhou | M.J. Liu

43. STUDIES ON SEED GERMINATION OF TUNISIAN JUJUBES

A. Laamouri | Y. Ammari | A. Albouchi | A. Dachraoui | M.T. Yakoubi

44. PROPAGATION STUDIES IN BER FOR COMMERCIAL MULTIPLICATION IN NURSERY AND IN SITU

S.N. Ghosh

45. SECONDARY METABOLITES FROM TISSUE CULTURES AND PLANT PARTS OF ZIZYPHUS MAURITIANA CULTIVARS GOLA AND SEB

T.N. Nag | N. Chouhan

46. FLORAL BIOLOGY STUDY OF ZIZIPHUS LOTUS L.

M.B. Nasri-Ayachi | M.A. Nabli

47. EFFECT OF DIFFERENT CULTURAL TREATMENTS ON YIELD AND PHYSICAL CHARACTERISTICS OF ZIZIPHUS JUJUBA MILL. GROWN IN CZECH REPUBLIC

S. Mishra | B. Krška

48. BLOSSOM AND FRUIT DROP AND ANATOMIC OBSERVATIONS ON EMBRYONIC DEVELOPMENT OF ZIZYPHUS JUJUBA MILL. CV. HUNANJIDANZAO

D.Y. Yuan | S. Wang | Z.Y. Gu | J.H. Duan | F.D. Li

49. EFFECT OF PLANT GROWTH REGULATORS ON FRUIT RETENTION, YIELD AND PHYSICO-CHEMICAL CHARACTERISTICS OF FRUITS IN BER ‘BANARASI KARKA’ GROWN IN CLOSE SPACING

S.N. Ghosh | B. Bera | A. Kundu | S. Roy

50. FRUIT PRODUCTION AND QUALITY IMPROVEMENT IN BANARASI KARKA CULTIVAR OF BER THROUGH CANOPY MANAGEMENT

S.N. Ghosh

51. THE DETERMINATION OF CHILLING REQUIREMENT AND CHANGES OF CARBOHYDRATE DURING DORMANCY FOR CHINESE JUJUBE

J.J. Du

52. STUDIES OF ARBUSCULAR MYCORRHIZAE ON THE GROWTH AND ABSORPTION OF PHOSPHORUS AND ZINC FROM THE SOIL FOR WILD JUJUBE (ZIZYPHUS SPINOSUS HU) SEEDLINGS

Y.M. Mao | L.Y. Shen | X.L. Wang | J.Y. Lu

53. STATUS OF DISEASES ON BER (ZIZIPHUS MAURITIANA LAMARCK) IN INDIA AND THEIR MANAGEMENT OPTIONS

M.M. Jamadar | R.A. Balikai | A.R. Sataraddi

54. GENETIC DIVERSITY OF TWENTY THREE STRAINS OF ZIZIPHUS JUJUBA MILL. CV. JUNZAO WITH DIFFERENT LEVELS OF RESISTANCE TO JUJUBE WITCHES’ BROOM DISEASE

Y. Qiao | J. Zhao | T.Y. Wu | Y.R. Yang | M.J. Liu

55. VARIATION OF MINERAL ELEMENT CONTENTS IN CHINESE JUJUBE WITH WITCHES’ BROOM DISEASE

J. Zhao | M.J. Liu

56. THE VIABILITY OF JUJUBE WITCHES’ BROOM (JWB) PHYTOPLASMA IN BRANCHES DURING WINTER AND THE NECESSITY OF ROOTS IN DEVELOPING JWB SYMPTOM IN CHINESE JUJUBE

J. Zhao | L. Dai | M.J. Liu

57. IDENTIFICATION OF RESISTANT CULTIVAR FOR JUJUBE WITCHES’ BROOM DISEASE AND DEVELOPMENT OF MANAGEMENT STRATEGIES

J. Zhao | M.J. Liu | X.Y. Liu | Z.H. Zhao

58. STRUCTURAL RESISTANCE OF DIFFERENT ECOTYPES OF ZIZIPHUS JUJUBA MILL. ‘JINSIXIAOZAO’ TO THICK ROTTEN DISEASE

W.C. Zhen | S.T. Wang | Y.N. Wang | C.Q. Liu* | Q.L. Wang

59. STUDY ON THE SYMPTOM, HARMFULNESS, AND THE KEY FACTOR FOR EPIDEMIC OF BLACK-SPOT DISEASE IN ZIZIPHUS JUJUBA ‘DONGZAO’

W.C. Zhen | S.T. Wang | Z.J. Jin | K.Q. Cao* | C.Q. Liu

60. SPATIAL DISTRIBUTION PATTERN OF OVER-WINTERING EGGS AND FIRST GENERATION OF NYMPHS AND ECOLOGICAL CONTROL OF LYGUS LUCORUM MEYER DÜR IN JUJUBE ORCHARD

61. STUDIES ON PARASITISM OF FOPIUS CARPOMYIE (SILVESTRI) (HYMENOPTERA: BRACONIDAE), AN EGG-PUPAL PARASITOID OF BER (KONAR) FRUIT FLY, CARPOMYIA VESUVIANA COSTA (DIPTERA: TEPHRITIDAE), IN BUSHEHR – IRAN

N. Farrar | R. Golestaneh | H. Askari | M.H. Assareh

62. EPIDEMIOLOGICAL STUDIES ON POWDERY MILDEW OF BER, ZIZIPHUS MAURITIANA LAMARCK

R.G. Jat | S.K. Goyal

63. FORECASTING OF POWDERY MILDEW DISEASE INCIDENCE ON BER (ZIZIPHUS MAURITIANA LAM.) BASED ON WEATHER

M.M. Jamadar | H. Venkatesh | R.A. Balikai | D.R. Patil

64. SEASONAL OCCURRENCE OF BER FRUIT WEEVIL, AUBEUS HIMALAYANUS VOSS IN INDIA

R.A. Balikai

65. INSECT PEST STATUS OF BER (ZIZIPHUS MAURITIANA LAMARCK) IN INDIA AND THEIR MANAGEMENT STRATEGIES

R.A. Balikai

66. POSTHARVEST DISEASE OF LINGWU CHANGZAO (ZIZIPHUS JUJUBA MILL.) FRUIT AND THE EFFECT OF BIOCONTROL AGENT ON FRUIT DECAY

H.L. Guo | B.Y. Wang | L. Li | Y.F. Hu

67. PRIMARY STUDY ON RESPIRATION TYPE OF LINGWU CHANGZAO (ZIZIPHUS JUJUBA MILL.)

68. EFFECT OF ULTRA HIGH PRESSURE ON POLYPHENOLOXIDASE OF JUJUBE FRUIT

G.Y. Zhao | Q.P. Xu

69. EFFECT OF ULTRA HIGH PRESSURE ON SOFTENING OF FRESH CUT JUJUBE FRUIT DURING STORAGE

W. Zong | G.J. An

70. EFFECT OF 1-MCP ON TEXTURE PROPERTIES OF FRESH FRUIT IN STORAGE SHELF PERIOD OF ZIZYPHUS JUJUBA ‘ZHONGQIUSUCUI’

71. EFFECTS OF FREEZING METHODS AND STORAGE TEMPERATURES ON THE FLESH FIRMNESS OF JUJUBE FRUITS

Z.H. Wang | J. Xue | L.P. Liu | X.M. Deng | T.J. Wei

72. EFFECTS OF CITRIC ACID TREATMENTS ON THE POSTHARVEST FRUIT QUALITY OF ZIZIPHUS JUJUBA MILL. ‘LINYILIZAO’

73. SUITABLE TEMPERATURE AND O₂/CO₂ COMPOSITION FOR FRESH FRUIT STORAGE OF ZIZIPHUS JUJUBA ‘DONGZAO’

H.Y. Sun | Y.Z. Wang | L. Yang | S.Y. Li

74. THE NUTRITIONAL AND PHYSICO-CHEMICAL PROPERTIES OF RIPE (ZIZIPHUS JUJUBE) FRUITS GROWN IN ISTRIA

R. Vidrih | N.P. Ulrih | E. Zlatić | J. Hribar | Z. Prgomet

75. CONTENT VARIATIONS OF WATER SOLUBLE POLYSACCHARIDES DURING FRUIT DEVELOPMENT IN CHINESE JUJUBE (ZIZIPHUS JUJUBA MILL.)

76. STUDY ON THE CONTENT OF TRITERPENE ACIDS AND FLAVONES FOR JUJUBES OF DIFFERENT CULTIVARS, DIFFERENT GROWING STAGES AND DIFFERENT PARTS

77. EXTRACTION AND SEPARATION OF CAMP FROM ZIZYPHUS JUJUBA FRUIT

Y.M. Chang | J.J. Du

78. STUDY ON THE ANTIOXIDANTS AND TOTAL PHENOL CONTENT OF OPTIMALLY SELECTED JUJUBE CULTIVARS

79. STUDY ON FRUIT QUALITY OF JUJUBE VARIETIES DURING MATURITY

R.P. He | J. Li | F. Zhao | W.N. Kong | R.S. Niu

80. SENSORY EVALUATION OF DIFFERENT PRODUCTS OF ZIZIPHUS JUJUBA MILL.

B. Krška | S. Mishra