Articles
DEVELOPMENT OF SOMATIC EMBRYO AND ADVENTITIOUS BUDS FROM THE DIFFERENTIATING CULTURED CALLI OF CHINESE JUJUBE MU-ZAO
Article number
840_36
Pages
273 – 282
Language
English
Abstract
To date, there was few data about the calli development of Chinese date (Chinese jujube) Mu-Zao in North of Shaanxi province.
Using the young tender leaves from Chinese date Mu-Zao in North of Shaanxi province as explants for tissue culture, the calli were induced from leaves by inoculated in MS or 3/4 MS medium with the affixture 6-BA 1.0 mg.L-1, IBA 0.2 mg.L-1, TDZ 0.015 mg.L-1, agar 0.55% and sugar 3%. Then, the induced calli were transferred into CYI basic medium with the affixture 6-BA 1.0 mg.L-1, IBA 0.2 mg.L-1, TDZ 0.015 mg.L-1, agar 0.55% and sugar 3% for the differentiating culture.
After cultured continuously for 40 d, the somatic embryos were developed.
The histological observations showed that Mu-Zao leaf somatic embryoids could be derived from a single somatic embryonic cell, the embryo sac mother cell or a cluster of embryonic cells.
Moreover, the development of Mu-Zao leaf somatic embryoids underwent different stages including a single embryonic cell, and then came through two cell embryo, quadrant embryo, many cell embryo, globular-shaped, heart-shaped, torpedo-shaped, mature embryo and budded stages.
During the development process, the conducting tissue could be observed evidently in the area concentrating with embryonic cells, and new-formed meristem in calli was also observed.
The active regions of occurrence of somatic embryogenesis and meristem were at surface layer of calli.
Therefore, cultured with the selective medium, the calli and meristems could be induced from the tender leaves of Chinese date Mu-Zao in North of Shaanxi province.
The development process of the cultured Mu-Zao somatic embryo was similar to that of zygotic embryo of discotyledonary plants.
These results may helpful for us to modify and create new date plants from North of Shaanxi province, P.R. China.
Using the young tender leaves from Chinese date Mu-Zao in North of Shaanxi province as explants for tissue culture, the calli were induced from leaves by inoculated in MS or 3/4 MS medium with the affixture 6-BA 1.0 mg.L-1, IBA 0.2 mg.L-1, TDZ 0.015 mg.L-1, agar 0.55% and sugar 3%. Then, the induced calli were transferred into CYI basic medium with the affixture 6-BA 1.0 mg.L-1, IBA 0.2 mg.L-1, TDZ 0.015 mg.L-1, agar 0.55% and sugar 3% for the differentiating culture.
After cultured continuously for 40 d, the somatic embryos were developed.
The histological observations showed that Mu-Zao leaf somatic embryoids could be derived from a single somatic embryonic cell, the embryo sac mother cell or a cluster of embryonic cells.
Moreover, the development of Mu-Zao leaf somatic embryoids underwent different stages including a single embryonic cell, and then came through two cell embryo, quadrant embryo, many cell embryo, globular-shaped, heart-shaped, torpedo-shaped, mature embryo and budded stages.
During the development process, the conducting tissue could be observed evidently in the area concentrating with embryonic cells, and new-formed meristem in calli was also observed.
The active regions of occurrence of somatic embryogenesis and meristem were at surface layer of calli.
Therefore, cultured with the selective medium, the calli and meristems could be induced from the tender leaves of Chinese date Mu-Zao in North of Shaanxi province.
The development process of the cultured Mu-Zao somatic embryo was similar to that of zygotic embryo of discotyledonary plants.
These results may helpful for us to modify and create new date plants from North of Shaanxi province, P.R. China.
Publication
Authors
Z.L. Chen, X.J. Wang, S.P. Liu, W. Cao, X.J. Ning, X.Y. Qi, X.Q. Zhang
Keywords
Zizyphus jujuba Mill., callus, differentiating culture, somatic embryogenesis, adventitious buds
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