TISSUE CULTURE OF CHINESE JUJUBE USING DIFFERENT EXPLANTS

The tissue culture of Chinese jujube (Ziziphus jujuba Mill.) was studied with different explants including stem tip, stem segment, leaves and embryo.
The results showed that: (1) MS medium could be used as basal medium for all above mentioned explants in Chinese jujube; (2) The optimum kinds and concentrations of growth regulators in medium were quite different among explants.
High concentration of 6-BA (about 0.5-3.0 mg/L) plus low concentration of IBA (about 0.1-0.5 mg/L) were suitable for initiation and proliferation culture of stem tip and stem segment.
TDZ of 1.0 mg/L was best for shoot regeneration from leaves.
No or low concentration of growth regulator (0.1 mg/L 6-BA+0.5 mg/L IBA+0.1 mg/L NAA) was fitted for 20-70 days old embryo culture; and (3) 6-BA of low concentration (0-0.1 mg/L) and IBA of high concentration (about 0.5-1.5 mg/L) or IAA of 1.0 mg/L were suitable for rooting culture of plantlet originated from different kinds of explants.
The tissue culture systems could be used as both solution for micropropagation and in vitro research platform for a series of basic and applied research in Chinese jujube.

I International Jujube Symposium

L. Dai, J. Zhao, M.J. Liu

Chinese jujube, tissue culture, stem tip and stem segment, leaves, embryo

https://doi.org/10.17660/ActaHortic.2009.840.39