Articles
DIRECT SHOOT REGENERATION FROM LEAF EXPLANTS OF SOUR JUJUBE
Article number
840_38
Pages
287 – 292
Language
English
Abstract
The direct shoot regeneration from leaf explants obtained from adult plantlets of sour jujube is reported.
Young leaves excised from plantlets of sour jujube were cultured on different medium compositions based on half of Murashige and Skoog (1962) salts.
Highest shoot regeneration frequency of 88.12% occurred on shoot inducing medium supplemented with benzylaminopurine (BA) (1.0 mg L-1) and silver nitrate (AgNO3) (1.0 mg L-1) within 3 weeks in the dark.
Medium devoid of AgNO3 and supplemented with only BA did not induce shoot regeneration.
The regenerated shoots were transferred to medium supplemented with 1.0 mg L-1 BA and 0.5 mg L-1 indole-3-butyric acid (IBA) for growth.
The shoots about 2 cm in height were transferred to rooting medium.
Subsequently profuse rooting of shoots was observed.
The good rooting percentage and root quality (96.56%, 4.82 roots shoot-1) were achieved on half of MS medium supplemented with 1.0 mg L-1 IBA. Complete plantlets were recovered within 7 weeks.
The plantlets were hardened and transferred to soil.
This developed direct plant regeneration method can be used for rapid mass cloning and genetic transformation and polyploidization of sour jujube.
Young leaves excised from plantlets of sour jujube were cultured on different medium compositions based on half of Murashige and Skoog (1962) salts.
Highest shoot regeneration frequency of 88.12% occurred on shoot inducing medium supplemented with benzylaminopurine (BA) (1.0 mg L-1) and silver nitrate (AgNO3) (1.0 mg L-1) within 3 weeks in the dark.
Medium devoid of AgNO3 and supplemented with only BA did not induce shoot regeneration.
The regenerated shoots were transferred to medium supplemented with 1.0 mg L-1 BA and 0.5 mg L-1 indole-3-butyric acid (IBA) for growth.
The shoots about 2 cm in height were transferred to rooting medium.
Subsequently profuse rooting of shoots was observed.
The good rooting percentage and root quality (96.56%, 4.82 roots shoot-1) were achieved on half of MS medium supplemented with 1.0 mg L-1 IBA. Complete plantlets were recovered within 7 weeks.
The plantlets were hardened and transferred to soil.
This developed direct plant regeneration method can be used for rapid mass cloning and genetic transformation and polyploidization of sour jujube.
Publication
Authors
N. Wang, M.J. Liu, Z.H. Zhao, Z.Y. Qin
Keywords
sour jujube (Z. acidojujube C.Y. Cheng et M.J. Liu), leaf explants, direct shoot regeneration, hardened plantlets
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