Articles
SECONDARY METABOLITES FROM TISSUE CULTURES AND PLANT PARTS OF ZIZYPHUS MAURITIANA CULTIVARS GOLA AND SEB
Article number
840_45
Pages
327 – 334
Language
English
Abstract
Plant growing in the xeric and harsh environment of desert produce various types of secondary metabolites which not only play a part in defence against drought, salinity and pathogens but may also serve as an excellent source of bioactive metabolites such as flavonoids, alkaloids, steroids etc.
Therefore, a screening of plants of arid areas both in vivo and in vitro for useful metabolites have been conducted in our laboratory.
Of the twenty one plants investigated Zizyphus mauritiana (ber) cultivars Gola and Seb gave excellent results.
Tissue cultures of both the cultivars were raised from leaves excised from mature plants and established for eighteen months by frequent sub-culturing on Murashige and Skoogs (1962) medium supplemented with cytokinins.
The presence of quercetin, kaempferol, sitosterol, stigmasterol, lanosterol and diosgenin in tissue cultures of both the cultivars were confirmed using TLC, MP, MMP and IR spectral analysis.
Therefore, a screening of plants of arid areas both in vivo and in vitro for useful metabolites have been conducted in our laboratory.
Of the twenty one plants investigated Zizyphus mauritiana (ber) cultivars Gola and Seb gave excellent results.
Tissue cultures of both the cultivars were raised from leaves excised from mature plants and established for eighteen months by frequent sub-culturing on Murashige and Skoogs (1962) medium supplemented with cytokinins.
The presence of quercetin, kaempferol, sitosterol, stigmasterol, lanosterol and diosgenin in tissue cultures of both the cultivars were confirmed using TLC, MP, MMP and IR spectral analysis.
Publication
Authors
T.N. Nag, N. Chouhan
Keywords
Zizyphus mauritiana cultivars, callus cultures, secondary metabolites, flavonoids, lanosterol, β-sitosterol, stigmasterol, diosgenin
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