Articles
THE PHYLOGENETIC RELATIONSHIPS OF GENUS ZIZIPHUS MILL. NATIVE TO CHINA BASED ON RAPD ANALYSIS
Article number
840_12
Pages
107 – 116
Language
English
Abstract
The genomic DNA variations of 14 species of Chinese Ziziphus, 12 cultivars of Z. jujuba Mill. and Paliurus spina-christi Mill. as outgroup were analyzed by random amplified polymorphic DNA (RAPD) technique.
Nine hundred nineteen bands (99.78%) were polymorphic out of 921 RAPD bands amplified by 31 random primers.
The data of 921 RAPD bands were used to generate Dices similarity coefficients and to construct a dendrogram.
On the basis of RAPD analysis Ziziphus species used in this study were grouped into six clusters with the genetic similarity coefficient of 0.26. The first cluster included Z. pubinervis Rehd.; The second one Z. oenoplia (L.) Mill. and Z. laui Merr.; The third one Z. incurva Roxb.; The fourth one Z. mairei Dode, Z. xiangchengensis Y.L. Chen et P.K. Chou, Z. montana W.W. Smith, Z. jujuba Mill., Z. acidojujuba C.Y. Cheng et M.J. Liu, the other cultivars of Z. jujuba Mill. and one outgroup P. spina-christi Mill.; The fifth one Z. mauritiana Lam., Z. fungii Merr., Z. rugosa Lam.; and The sixth one Z. attopensis Pierre. and Z. apetala Hook. f.
Based on this analysis, revision in the current taxonomy and new classification in Chinese Ziziphus has been proposed.
Nine hundred nineteen bands (99.78%) were polymorphic out of 921 RAPD bands amplified by 31 random primers.
The data of 921 RAPD bands were used to generate Dices similarity coefficients and to construct a dendrogram.
On the basis of RAPD analysis Ziziphus species used in this study were grouped into six clusters with the genetic similarity coefficient of 0.26. The first cluster included Z. pubinervis Rehd.; The second one Z. oenoplia (L.) Mill. and Z. laui Merr.; The third one Z. incurva Roxb.; The fourth one Z. mairei Dode, Z. xiangchengensis Y.L. Chen et P.K. Chou, Z. montana W.W. Smith, Z. jujuba Mill., Z. acidojujuba C.Y. Cheng et M.J. Liu, the other cultivars of Z. jujuba Mill. and one outgroup P. spina-christi Mill.; The fifth one Z. mauritiana Lam., Z. fungii Merr., Z. rugosa Lam.; and The sixth one Z. attopensis Pierre. and Z. apetala Hook. f.
Based on this analysis, revision in the current taxonomy and new classification in Chinese Ziziphus has been proposed.
Publication
Authors
L. Li, J.Y. Peng, R.X. Bai, R.H. Huang, B.Q. Zheng
Keywords
Ziziphus, phylogenetic relationship, RAPD
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