Articles
IDENTIFICATION OF PLUM X APRICOT INTERSPECIFIC HYBRIDS USING ISOENZYME POLYMPRPHISM
Article number
488_57
Pages
361 – 368
Language
Abstract
The purpose of this work was to find isoenzyme markers for plum x apricot (plumcot) hybrids to uniquely identify hybrid seedlings from plum x apricot crosses.
Ten Japanese plum cultivars (Prunus salicina), 17 apricot cultivars (P. armeniaca), seven plum x apricot (plumcot) hybrids, which were derived from the breeding program of Agricultural Research Service in Fresno and two complex hybrids (Pluot and Aprium) were examined electrophoretically.
Starch gels of three buffer systems and acrylamide gels were used for electrophoretic separation of twenty enzyme systems.
Ten Japanese plum cultivars (Prunus salicina), 17 apricot cultivars (P. armeniaca), seven plum x apricot (plumcot) hybrids, which were derived from the breeding program of Agricultural Research Service in Fresno and two complex hybrids (Pluot and Aprium) were examined electrophoretically.
Starch gels of three buffer systems and acrylamide gels were used for electrophoretic separation of twenty enzyme systems.
The most informative enzymes for identifying plumcots were peroxidase (PRX-2, PRX-Cathodal), acid phosphatase (ACP-2) and shikimate dehydrogenase (SKD-1, SKD-2). In addition superoxide dismutase bands SOD-1c and SOD-1d and phosphoglucomutase PGM-2b showed activity only in plums and in plumcots.
The existing polymorphism allows to distinguish individually all seven examined plumcots.
Publication
Authors
A.G. Manganaris, A. Mainou, A. Goudaras, C. Ledbetter
Keywords
Isoenzymes, apricots, plums, interspecific hybrids, identification
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