Articles
Developing new cultivars of yellow hybrid lily and propagation of its planting materials
Article number
1334_11
Pages
87 – 94
Language
English
Abstract
The demand for lily flowers and propagation materials in Indonesia has been addressed through import.
To meet this growing demand, it is necessary to develop locally new lily cultivars that are adaptive to grow on the local climate conditions.
This research was conducted at the Laboratory and Experimental Station of the Indonesian Ornamental Plants Research Institute (IOCRI) in 2017-2019. The BC1 population was formed from F1 plants (Ceb Glow × FO-14T) crossed with Ceb Glow as males.
All individuals in the BC1 population were assessed quantitatively and qualitatively.
The morphological and growth analysis showed that the number of flowers and plant height seems to be genetically controlled, but the flower diameter is not.
There was a close relationship between the length of the sepals and the length of the petals, the diameter of the tubers and the number of flowers, the diameter of the stems and the number of flowers, and the diameter of the tubers and the diameter of the trunk.
Individual hybrid lilies with 18 flowers per stalk were propagated in vitro.
Flower buds were used as explants cultured in MS medium supplemented with 3 plant growth regulator compositions.
The best medium was ¾ MS enriched with thidiazuron, benzyl adenine and naphthalene acetic acid, each with a concentration of 0.1 ppm.
Callus was formed at the base of the tepal within 2 weeks.
After two weeks, the callus had regenerated into bulblets, which were then grown in MS medium without hormones.
Within 4-6 months tubers with a diameter of 2.3-3 cm were formed.
The tubers were stored in cool storage and distributed to stakeholders.
To meet this growing demand, it is necessary to develop locally new lily cultivars that are adaptive to grow on the local climate conditions.
This research was conducted at the Laboratory and Experimental Station of the Indonesian Ornamental Plants Research Institute (IOCRI) in 2017-2019. The BC1 population was formed from F1 plants (Ceb Glow × FO-14T) crossed with Ceb Glow as males.
All individuals in the BC1 population were assessed quantitatively and qualitatively.
The morphological and growth analysis showed that the number of flowers and plant height seems to be genetically controlled, but the flower diameter is not.
There was a close relationship between the length of the sepals and the length of the petals, the diameter of the tubers and the number of flowers, the diameter of the stems and the number of flowers, and the diameter of the tubers and the diameter of the trunk.
Individual hybrid lilies with 18 flowers per stalk were propagated in vitro.
Flower buds were used as explants cultured in MS medium supplemented with 3 plant growth regulator compositions.
The best medium was ¾ MS enriched with thidiazuron, benzyl adenine and naphthalene acetic acid, each with a concentration of 0.1 ppm.
Callus was formed at the base of the tepal within 2 weeks.
After two weeks, the callus had regenerated into bulblets, which were then grown in MS medium without hormones.
Within 4-6 months tubers with a diameter of 2.3-3 cm were formed.
The tubers were stored in cool storage and distributed to stakeholders.
Authors
L.L. Sanjaya, B. Marwoto, R. Indrasti, I.B. Raharjo, R. Kurniati
Keywords
backcross, in vitro culture, number of flowers, flower diameter, hybrids
Groups involved
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