Articles
Time-course transcriptome analysis of leaf cutting provides an insight into the mechanism of Zamioculcas zamiifolia vegetative regeneration
Article number
1334_28
Pages
229 – 240
Language
English
Abstract
Zamioculcas zamiifolia is a foliage plant belonging to the Araceae family and has evolved a unique asexual regeneration mechanism that a newborn shoot developed from a tuber-like body with high water content.
To explore this unique mechanism of asexual regeneration of this plant, leaves without cutting were used as control, the petioles of the cutting leaves were sampled at the 6th and 15th day after cutting to extract RNA and sequenced using Illumina Hiseq platform.
Time-course transcriptome sequencing generated over 7.57 Gb the clean data for each sample and assembled 88,331 unigenes.
Total 33,554 unigenes were annotated by NR, Swiss-Prot, COG, KOG, eggNOG. As many as 3,340 differential expressed genes were found in the regeneration initial stage, of which 2,198 were upregulated genes and 1,142 downregulated genes.
Differentially expressed genes of 4,070 were screened out in the tuber formation initial stage including 2,814 upregulated genes and 1,260 downregulated genes.
There were 1,500 differentially expressed genes in the sequence profile between regeneration initial and tuber formation with 993 upregulated genes and 507 downregulated genes.
Three sequencing profiles share 296 differentially expressed genes.
The function of 161 genes annotated by GO is involved in molecular functions, biological processes, and cell components.
KEGG annotation total 31 metabolic pathways which include the glutathione metabolic pathway, starch versus sucrose metabolic pathway and phytohormone signaling transduction pathway were with the most enrichment.
The candidate differentially expressed genes of 24 were predicted and might play an important role in regeneration initiation and tuber-like body induction of Zamioculcas zamiifolia cutting.
To explore this unique mechanism of asexual regeneration of this plant, leaves without cutting were used as control, the petioles of the cutting leaves were sampled at the 6th and 15th day after cutting to extract RNA and sequenced using Illumina Hiseq platform.
Time-course transcriptome sequencing generated over 7.57 Gb the clean data for each sample and assembled 88,331 unigenes.
Total 33,554 unigenes were annotated by NR, Swiss-Prot, COG, KOG, eggNOG. As many as 3,340 differential expressed genes were found in the regeneration initial stage, of which 2,198 were upregulated genes and 1,142 downregulated genes.
Differentially expressed genes of 4,070 were screened out in the tuber formation initial stage including 2,814 upregulated genes and 1,260 downregulated genes.
There were 1,500 differentially expressed genes in the sequence profile between regeneration initial and tuber formation with 993 upregulated genes and 507 downregulated genes.
Three sequencing profiles share 296 differentially expressed genes.
The function of 161 genes annotated by GO is involved in molecular functions, biological processes, and cell components.
KEGG annotation total 31 metabolic pathways which include the glutathione metabolic pathway, starch versus sucrose metabolic pathway and phytohormone signaling transduction pathway were with the most enrichment.
The candidate differentially expressed genes of 24 were predicted and might play an important role in regeneration initiation and tuber-like body induction of Zamioculcas zamiifolia cutting.
Authors
Z.W. Hou, H. Zhang, S.L. Yang, W.Y. He, X.Y. Li, M.L. Wang, W. Jin, Y.Y. Zheng, L.L. Gao, T.X. Zhong, F.X. Liao
Keywords
RNA-seq, differentially expressed genes, Araceae, asexual regeneration
Groups involved
Online Articles (49)