Articles
Somatic embryogenesis of Aquilaria malaccensis Lamk
Article number
1334_26
Pages
215 – 220
Language
English
Abstract
Aquilaria malaccensis belongs to the Thymelaeaceae family and is known as agarwood in the world and gaharu in Malaysia.
It is the most important plant that is commercially identified as a potential aromatic plant in fragrance industries.
Conventional inoculation for resinous compounds in A. malaccensis is difficult.
Hence, in this study, a somatic embryogenesis method was developed.
Stem explants of A. malaccensis were cultured on MS media supplemented with BAP and 2,4-D for the callus induction.
Embryogenic callus was successfully initiated on MS media supplemented with 1.0 mg L‑1 BAP and 0.1 mg L‑1 2,4-D added with 500 mg L‑1 casein hydrolysate (CH) producing globular, heart-shaped, and torpedo stages.
Addition of additives including CH added with 10.0 mg L‑1 abscisic acid (ABA) gave a significant effect on the development and maturation of embryogenic callus.
Therefore, the induction of a high frequency of somatic embryogenesis in stem explants on MS media is possible.
Optimum embryogenic callus formation was achieved from MS media added with 500 mg L‑1 CH. A reliable protocol useful for A. malaccensis through somatic embryogenesis was successfully established for plant tissue culture purposes.
It is the most important plant that is commercially identified as a potential aromatic plant in fragrance industries.
Conventional inoculation for resinous compounds in A. malaccensis is difficult.
Hence, in this study, a somatic embryogenesis method was developed.
Stem explants of A. malaccensis were cultured on MS media supplemented with BAP and 2,4-D for the callus induction.
Embryogenic callus was successfully initiated on MS media supplemented with 1.0 mg L‑1 BAP and 0.1 mg L‑1 2,4-D added with 500 mg L‑1 casein hydrolysate (CH) producing globular, heart-shaped, and torpedo stages.
Addition of additives including CH added with 10.0 mg L‑1 abscisic acid (ABA) gave a significant effect on the development and maturation of embryogenic callus.
Therefore, the induction of a high frequency of somatic embryogenesis in stem explants on MS media is possible.
Optimum embryogenic callus formation was achieved from MS media added with 500 mg L‑1 CH. A reliable protocol useful for A. malaccensis through somatic embryogenesis was successfully established for plant tissue culture purposes.
Authors
N.N. Ibrahim, A. Awal, N.A. Yusuf, N.J. Sidik, A.M. Salahbiah, B. Satria
Keywords
plant tissue culture, somatic embryogenesis, Aquilaria malaccensis
Groups involved
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