Articles
Advancements in micropropagation of Cannabis sativa L. via temporary immersion systems
Article number
1454_16
Pages
115 – 122
Language
English
Abstract
Cannabis sativa L., a plant of significant cultural importance for thousands of years, has recently seen a surge in demand within the pharmaceutical and cosmetic sectors for its flowers.
Traditionally harvested from clonally propagated female plants, these flowers are rich sources of cannabinoids and other valuable secondary metabolites.
Addressing this demand, our research explores the potential of temporary immersion systems (TIS) to streamline and enhance the in vitro micropropagation process.
This study rigorously evaluated the effectiveness of TIS across four genetically distinct medicinal cannabis genotypes, each representative of a different chemotype (chemotypes I to IV). We assessed the success of propagation by analyzing various parameters: the number of shoots generated, their lengths, and degrees of hyperhydration, chlorophyll content along with other critical morphological traits.
A comparative analysis was also conducted with plants grown under standard micropropagation technique, involving nodal segments cultured in solid medium within test tubes.
Results indicate that TIS markedly enhances the efficiency of cannabis micropropagation, suggesting that it could be effectively scaled up for commercial applications.
This research not only supports the continued optimisation of TIS parameters but also contributes to a deeper understanding of its impact on the epigenetic and physiological characteristics of Cannabis sativa, aiming to optimise the production of secondary metabolites.
Traditionally harvested from clonally propagated female plants, these flowers are rich sources of cannabinoids and other valuable secondary metabolites.
Addressing this demand, our research explores the potential of temporary immersion systems (TIS) to streamline and enhance the in vitro micropropagation process.
This study rigorously evaluated the effectiveness of TIS across four genetically distinct medicinal cannabis genotypes, each representative of a different chemotype (chemotypes I to IV). We assessed the success of propagation by analyzing various parameters: the number of shoots generated, their lengths, and degrees of hyperhydration, chlorophyll content along with other critical morphological traits.
A comparative analysis was also conducted with plants grown under standard micropropagation technique, involving nodal segments cultured in solid medium within test tubes.
Results indicate that TIS markedly enhances the efficiency of cannabis micropropagation, suggesting that it could be effectively scaled up for commercial applications.
This research not only supports the continued optimisation of TIS parameters but also contributes to a deeper understanding of its impact on the epigenetic and physiological characteristics of Cannabis sativa, aiming to optimise the production of secondary metabolites.
Authors
L. Bonin, N. Pelko, T. Kvasič, J. Murovec
Keywords
chemotype, plant tissue culture, secondary metabolites, clonal propagation, pharmaceutical applications, waste reduction
Groups involved
- Division Plant Genetic Resources, Breeding and Biotechnology
- Working Group Genetic Transformation and Gene Editing
- Working Group Horticultural Biotechnology and Breeding
- Division Ornamental Plants
- Division Vegetables, Roots and Tubers
- Division Temperate Tree Fruits
- Division Vine and Berry Fruits
- Division Horticulture for Development
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