Articles
Ri technology offers potential for breeding for replant disease tolerance in rose and apple
Article number
1454_30
Pages
215 – 222
Language
English
Abstract
Replant disease (RD) causes severe growth and yield reduction in consecutive cultures of the same species, particularly affecting Rosaceae family members, with no sustainable management solutions currently available.
This study investigated whether the root-inducing (Ri) technology, which integrates bacterial rol genes into plant genomes, could enhance RD tolerance in apple and rose.
Nine apple and three rose Ri genotypes were tested alongside their wildtype genotypes in a greenhouse biotest using both RD-affected and gamma-irradiated (G) RD soil.
After eight weeks, all Ri genotypes displayed characteristic reduced shoot growth (21.9-75.5% in apple, 32.6-42.8% in rose) compared to wildtype genotypes in G soil.
When exposed to RD conditions, some Ri genotypes showed improved tolerance in terms of RD/G ratios of growth parameters.
Rose Ri genotypes exhibited a more pronounced shoot length increase during the biotest, with RD/G ratios ranging from 1.31 to 1.84, whereas the wildtype genotypes showed lower ratios of 0.90 and 0.46. One particular rose Ri genotype displayed significantly greater shoot fresh mass (0.87) and root fresh mass (1.20) ratios in comparison to its wildtype, which had notably lower ratios of 0.55 and 0.67, respectively.
Likewise, two apple Ri genotypes demonstrated significantly improved shoot fresh mass ratios of 0.57 and 0.61, as well as one genotype with a significantly higher root fresh mass ratio of 1.00, while the wildtype genotype M26 showed lower ratios for shoot fresh mass (0.37) and root fresh mass (0.52). This study demonstrates for the first time that Ri technology can enhance RD tolerance in Rosaceae crops, while the underlying mechanisms remain to be investigated.
These findings may pave the way for developing RD-tolerant varieties, offering a sustainable solution to a persistent problem in commercial horticulture.
This study investigated whether the root-inducing (Ri) technology, which integrates bacterial rol genes into plant genomes, could enhance RD tolerance in apple and rose.
Nine apple and three rose Ri genotypes were tested alongside their wildtype genotypes in a greenhouse biotest using both RD-affected and gamma-irradiated (G) RD soil.
After eight weeks, all Ri genotypes displayed characteristic reduced shoot growth (21.9-75.5% in apple, 32.6-42.8% in rose) compared to wildtype genotypes in G soil.
When exposed to RD conditions, some Ri genotypes showed improved tolerance in terms of RD/G ratios of growth parameters.
Rose Ri genotypes exhibited a more pronounced shoot length increase during the biotest, with RD/G ratios ranging from 1.31 to 1.84, whereas the wildtype genotypes showed lower ratios of 0.90 and 0.46. One particular rose Ri genotype displayed significantly greater shoot fresh mass (0.87) and root fresh mass (1.20) ratios in comparison to its wildtype, which had notably lower ratios of 0.55 and 0.67, respectively.
Likewise, two apple Ri genotypes demonstrated significantly improved shoot fresh mass ratios of 0.57 and 0.61, as well as one genotype with a significantly higher root fresh mass ratio of 1.00, while the wildtype genotype M26 showed lower ratios for shoot fresh mass (0.37) and root fresh mass (0.52). This study demonstrates for the first time that Ri technology can enhance RD tolerance in Rosaceae crops, while the underlying mechanisms remain to be investigated.
These findings may pave the way for developing RD-tolerant varieties, offering a sustainable solution to a persistent problem in commercial horticulture.
Authors
P. Rüter, M.H. Doan, T. Winkelmann
Keywords
Agrobacterium rhizogenes, apple replant disease, hairy roots, Rhizobium rhizogenes, rol genes, rose replant disease, stress tolerance
Groups involved
- Division Plant Genetic Resources, Breeding and Biotechnology
- Working Group Genetic Transformation and Gene Editing
- Working Group Horticultural Biotechnology and Breeding
- Division Ornamental Plants
- Division Vegetables, Roots and Tubers
- Division Temperate Tree Fruits
- Division Vine and Berry Fruits
- Division Horticulture for Development
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