The effect of different nutrient media on in vitro propagation of vulnerable Kostelekzya pentacarpos (L.) Ledeb.

The present study investigates the effects of different nutrient media and a low concentration of kinetin (KIN) on the in vitro propagation of Kosteletzkya pentacarpos, Seeds collected from native plants were germinated in vitro on half-strength Murashige and Skoog (MS) medium at three temperatures (15, 20, and 25°C), under a 16-h photoperiod.
Germination was significantly affected by temperature, with the highest percentage (76.1%) observed at 25°C. In vitro cultures were successfully established from single-node explants excised from aseptic seedlings, on MS medium either hormone free (Hf) or supplemented with 0.5 mg L^‑1 KIN, achieving 100% explant response.
Each explant produced 1.1-1.3 shoots, while the presence of KIN reduced the shoot length (11.0 and 7.5 cm on MS Hf and supplemented with 0.5 mg L^‑1 KIN, respectively). Subsequent subcultures were conducted on MS, Rugini Olive Medium (OM), Driver and Kuniyuki Basal Medium (DKW) and Woody Plant Medium (WPM), either Hf or supplemented with 0.5 mg L^‑1 KIN. The highest multiplication indices were observed in OM Hf or supplemented with 0.5 mg L^‑1 KIN and MS Hf media (23.3, 20.0, and 21.7, respectively). Micro-plants into OM Hf produced the longest shoots (14.6 cm). Spontaneous rooting occurred at 100% across all media tested.
These findings suggest that both OM and MS media are suitable for the in vitro establishment of K. pentacarpos subcultures.

International Symposium on Biotechnological Tools in Horticulture

K. Bertsouklis, D. Vazaka-Vodena, N. Ntoulas, E. Dima, S. Hatzilazarou, S. Kostas, M. Villani

aseptic seedlings, DKW, kinetin, Rugini Olive Medium, spontaneous rooting, tissue culture, WPM

https://doi.org/10.17660/ActaHortic.2026.1454.17