Articles
Effect of pretreatment media on adventitious shoot regeneration from leaves of plum (Prunus domestica ‘Sirma’)
Article number
1454_53
Pages
379 – 386
Language
English
Abstract
Breeding programs has been used to develop new plum cultivars characterized by high productivity, fruit quality, disease resistance, especially to plum pox virus.
In recent years, contemporary biotechnological approaches involving in vitro tissue culture, genetic transformation, molecular marker development etc. have an important role in plum breeding.
In most woody fruit species, however, adventitious regeneration and transformation are difficult, with low efficiency and often limited to a few genotypes or to seed derived tissues.
The aim of the present study was to evaluate the effect of pretreatment media on adventitious shoot regeneration from leaves of plum (Prunus domestica ‘Sirma’). The source plants were grown on nutrient media based on both Murashige and Skoog (MS) or Driver and Kuniyuki (DKW), supplemented with cytokinins – either benzylaminopurine (BAP) or meta-Topolin (mT) (2.5 or 5 µM). Leaves of the source plants were cultivated for regeneration on MS nutrient media with added 7.5 or 9 μM thidiazuron (TDZ). The explants were cultivated in darkness for 15 days, then under a 16/8 photoperiod (30 μmol m‑2 s‑1 PPFD) at 22±2°С for 28 days.
Among the different treatments studied the regeneration frequency varies between 18 and 64%. The best efficiency of somatic organogenesis (over 48% regeneration) was achieved on regeneration medium supplemented with 9 μM TDZ with leaf explants isolated from plantlets grown on either MS or DKW media with 5 µM mT. There is no proven complex influence of the three factors on the number of shoots of the studied factors.
In recent years, contemporary biotechnological approaches involving in vitro tissue culture, genetic transformation, molecular marker development etc. have an important role in plum breeding.
In most woody fruit species, however, adventitious regeneration and transformation are difficult, with low efficiency and often limited to a few genotypes or to seed derived tissues.
The aim of the present study was to evaluate the effect of pretreatment media on adventitious shoot regeneration from leaves of plum (Prunus domestica ‘Sirma’). The source plants were grown on nutrient media based on both Murashige and Skoog (MS) or Driver and Kuniyuki (DKW), supplemented with cytokinins – either benzylaminopurine (BAP) or meta-Topolin (mT) (2.5 or 5 µM). Leaves of the source plants were cultivated for regeneration on MS nutrient media with added 7.5 or 9 μM thidiazuron (TDZ). The explants were cultivated in darkness for 15 days, then under a 16/8 photoperiod (30 μmol m‑2 s‑1 PPFD) at 22±2°С for 28 days.
Among the different treatments studied the regeneration frequency varies between 18 and 64%. The best efficiency of somatic organogenesis (over 48% regeneration) was achieved on regeneration medium supplemented with 9 μM TDZ with leaf explants isolated from plantlets grown on either MS or DKW media with 5 µM mT. There is no proven complex influence of the three factors on the number of shoots of the studied factors.
Authors
L. Nacheva, N. Dimitrova, D. Aleksandrova, N. Keranova
Keywords
somatic organogenesis, somaclonal variation, pretreatment, meta-Тopolin, benzylaminopurine, thidiazuron, sorbitol
Groups involved
- Division Plant Genetic Resources, Breeding and Biotechnology
- Working Group Genetic Transformation and Gene Editing
- Working Group Horticultural Biotechnology and Breeding
- Division Ornamental Plants
- Division Vegetables, Roots and Tubers
- Division Temperate Tree Fruits
- Division Vine and Berry Fruits
- Division Horticulture for Development
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