Articles
Moracin M production from root cultures in mulberry (Morus alba L.)
Article number
1454_50
Pages
359 – 364
Language
English
Abstract
Mulberry (Morus alba L.) extracts contain numerous bioactive compounds with antioxidant and anti-inflammatory capacities.
In vitro culture is an endless source of plant material that can be used to produce many of these bioactive compounds.
In this study, in vitro root cultures of the ‘Filipina’ cultivar, previously selected on the basis of its high capacity to produce phenolic compounds, have been used to produce Moracin M, using a cyclodextrin as the elicitor.
Root tips of ‘Filipina’ were cultured in Woody Plant Medium supplemented with 1 mg L‑1 of naphthaleneacetic acid and 2-hydroxypropyl-beta-cyclodextrin (HP
CD) at the concentrations of 0, 10, 15, and 20 mM. Roots were cultured in darkness.
Aliquots from the culture medium were taken at 0, 24, 48, 72, 96, 168, 336, and 504 h after the culture was started, and Moracin M was measured.
Moracin M was found in higher amounts in the samples taken at 336 and 504 h in all of the treatments, except for the control.
On the other hand, media containing 20 mM of HPCD produced the highest content in Moracin M compared with the rest of the treatments.
In vitro culture is an endless source of plant material that can be used to produce many of these bioactive compounds.
In this study, in vitro root cultures of the ‘Filipina’ cultivar, previously selected on the basis of its high capacity to produce phenolic compounds, have been used to produce Moracin M, using a cyclodextrin as the elicitor.
Root tips of ‘Filipina’ were cultured in Woody Plant Medium supplemented with 1 mg L‑1 of naphthaleneacetic acid and 2-hydroxypropyl-beta-cyclodextrin (HP
CD) at the concentrations of 0, 10, 15, and 20 mM. Roots were cultured in darkness.Aliquots from the culture medium were taken at 0, 24, 48, 72, 96, 168, 336, and 504 h after the culture was started, and Moracin M was measured.
Moracin M was found in higher amounts in the samples taken at 336 and 504 h in all of the treatments, except for the control.
On the other hand, media containing 20 mM of HP
CD produced the highest content in Moracin M compared with the rest of the treatments.
Authors
F.J. Vidal-Sánchez, S.A. Álvarez, J.M. Gambín, C. López-Sierra, M. Romero-Muñoz, J.M. López-Nicolás, M. Pérez-Jiménez
Keywords
tissue culture, in vitro culture, ANA, HPβCD, elicitation
Groups involved
- Division Plant Genetic Resources, Breeding and Biotechnology
- Working Group Genetic Transformation and Gene Editing
- Working Group Horticultural Biotechnology and Breeding
- Division Ornamental Plants
- Division Vegetables, Roots and Tubers
- Division Temperate Tree Fruits
- Division Vine and Berry Fruits
- Division Horticulture for Development
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