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Articles

Characterization of extracellular vesicles in fall armyworm cells as potential transport of RNA: first data

Article number

1454_41

Pages

293 – 298

Language

English

Abstract

Spodoptera frugiperda (fall armyworm) is a major pest of agronomic and horticultural crops across tropical and subtropical regions.
This study presents the first evidence of extracellular vesicles (EVs) in this insect.
EVs, which mediate intercellular communication, were isolated from Sf9 cells using iodixanol-gradient ultracentrifugation.
Nanoparticle tracking analysis (NTA) and scanning electron microscopy (SEM) confirmed their presence.
Gel electrophoresis investigated dsRNA in EVs, investigating a potential role in extracellular RNA transport.

Publication

International Symposium on Biotechnological Tools in Horticulture

Authors

G. Smagghe, Jialing Liu, Yuqian Cheng, Dongdong Liu

Keywords

RNAi, Spodoptera frugiperda, Sf9 cells, dsRNA, extracellular vesicles, EVs

Full text

https://doi.org/10.17660/ActaHortic.2026.1454.41

Groups involved

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