Articles
Indirect plant regeneration of drought-resistant ecotypes of Lavandula latifolia Medik.
Article number
1454_49
Pages
351 – 358
Language
English
Abstract
One major concern in establishing non-domesticated aromatic and medicinal crops is the low germination rate exhibited by these species.
This is the case of spike lavender (Lavandula latifolia Medik.), which, despite being one of the most widely cultivated rainfed aromatic crops in Southeast Spain, is primarily propagated through seed germination techniques.
This reliance on seeds limits the economic potential of this aromatic crop, which serves as a supplemental income source for many rural areas in the western Mediterranean region.
Therefore, the primary objective of this work is to develop a novel propagation technique that enables the production of a substantial number of clones in a short timeframe.
To this end, explants from drought-resistant ecotypes, stored in the IMIDA bank of germplasm, were placed in MS medium containing 16 different combinations of plant growth regulators (auxins and cytokinins) to induce callus formation over a period of four weeks.
After this, all clustered calli were shaken in liquid media to separate the individual plantlets.
The separated plantlets were then planted in MS medium for regeneration.
At the end of the experiment, the method enabled the development of a protocol for regenerating plants from L. latifolia leaves, based on the results obtained from the most effective combinations of growth media and plant growth regulators (PGRs) during each stage of the process.
This is the case of spike lavender (Lavandula latifolia Medik.), which, despite being one of the most widely cultivated rainfed aromatic crops in Southeast Spain, is primarily propagated through seed germination techniques.
This reliance on seeds limits the economic potential of this aromatic crop, which serves as a supplemental income source for many rural areas in the western Mediterranean region.
Therefore, the primary objective of this work is to develop a novel propagation technique that enables the production of a substantial number of clones in a short timeframe.
To this end, explants from drought-resistant ecotypes, stored in the IMIDA bank of germplasm, were placed in MS medium containing 16 different combinations of plant growth regulators (auxins and cytokinins) to induce callus formation over a period of four weeks.
After this, all clustered calli were shaken in liquid media to separate the individual plantlets.
The separated plantlets were then planted in MS medium for regeneration.
At the end of the experiment, the method enabled the development of a protocol for regenerating plants from L. latifolia leaves, based on the results obtained from the most effective combinations of growth media and plant growth regulators (PGRs) during each stage of the process.
Authors
G. Cáceres-Cevallos, T. Lopes, M. Jordán, S. Correia
Keywords
Lavandula latifolia, aromatic-medicinal plants, indirect organogenesis, drought-resistant varieties
Groups involved
- Division Plant Genetic Resources, Breeding and Biotechnology
- Working Group Genetic Transformation and Gene Editing
- Working Group Horticultural Biotechnology and Breeding
- Division Ornamental Plants
- Division Vegetables, Roots and Tubers
- Division Temperate Tree Fruits
- Division Vine and Berry Fruits
- Division Horticulture for Development
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