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Articles

Isolation of extracellular vesicles and characterization of their role as shuttle of biologically active small RNAs in intercellular communication

Article number

1454_42

Pages

299 – 306

Language

English

Abstract

Cell-cell communication is crucial for the coordination of various biological processes in multicellular organisms and is mediated by multiple factors, such as hormones, peptides, and small non-coding RNAs.
More recently, another mode of intercellular communication has emerged through the encapsulation of biologically active components in extracellular vesicles (EVs). EVs have attracted interest not only as mediators of communication between neighboring cells, but also for their role in cross-kingdom communication between interacting organisms.
In this work, we present an optimized protocol for the isolation of EVs from Nicotiana benthamiana leaves and the preliminary characterization of the small RNA composition of EVs.

Publication

International Symposium on Biotechnological Tools in Horticulture

Authors

T. Sanson, M. Cecchin, B. Molesini, T. Pandolfini

Keywords

Full text

https://doi.org/10.17660/ActaHortic.2026.1454.42

Groups involved

Online Articles (75)

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3. Grapevine genome editing for improving disease resistance and quality traits

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4. A decision tree-based toolbox for multiplex CRISPR/Cas to modify complex traits – case studies in potato and chicory

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5. A CRISPR/Cas9 approach to improve the quality of tomato by reducing the content of allergens

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7. Octoploid strawberry repaired mutated sequences CRISPR/Cas9 edited

8. Intragenic and synthetic biotechnology for genetic improvement in strawberry

9. In-planta transformation in citrus using the RUBY system and developmental regulators

10. Editing approaches to improve stress tolerance in tomato

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12. Epigenetic variations in micropropagated horticultural crops and their prospects in crop improvement

S.C. Debnath

13. Efficient production of viroid-free German hops using meristem culture and M-bridge technique

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14. Thermotherapy: an efficient tool for pathogens elimination in apricot

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16. Advancements in micropropagation of Cannabis sativa L. via temporary immersion systems

L. Bonin | N. Pelko | T. Kvasič | J. Murovec

17. The effect of different nutrient media on in vitro propagation of vulnerable Kostelekzya pentacarpos (L.) Ledeb.

18. Experiments on the elimination of plum pox virus and Candidatus Phytoplasma prunorum in nectarine (Prunus persica (L.) Batsch. var. nucipersica)

L. Nacheva | S. Milusheva | M. Gozmanova | R. Valkova | I. Tsvetkov

19. Photoautotrophic micropropagation in maqui (Aristotelia chilensis)

H. Vogel | D. Bascuñán | V. Muñoz | B. González

20. Automatic image segmentation as a possible key to detect contamination in in vitro plant tissue culture

21. Improvement of the germination rate of Lavandula latifolia Medik. by application of chitosan nanoparticles

22. Chitosan nanoparticles improve germination in non-stratified peach (Prunus persica L. Batsch) seeds

23. Unraveling Psa resistance in kiwifruit: insights from A. chinensis and A. arguta gene expression

G. De Mori | F. Marroni | G. Cipriani

24. Genome sequencing of Monilinia spp. and expression of Monilinia fructicola putative effector genes during infection of peach (Prunus persica) cultivars ‘Royal Summer’ and ‘Messapia’

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26. Development of a detached leaf assay to evaluate Hydrangea macrophylla cultivars for susceptibility to Botrytis cinerea

E. Fatemi | L. Fazlikhani | J. Schumacher | F. Engel | C. Tränkner

27. Deciphering genetic and epigenetic drivers of degeneration in the Austrian grape cultivar ‘Neuburger’

R. Hack | F. Regner

28. A marker-trait association analysis to unlock the genetic determinism of tolerance towards bot gummosis disease in lemon

29. In vitro elicitation of olive calli with methyl jasmonate and Xylella fastidiosa lysate: effects on secondary metabolism and gene expression

30. Ri technology offers potential for breeding for replant disease tolerance in rose and apple

P. Rüter | M.H. Doan | T. Winkelmann

31. Use of Wickerhamomyces anomalus as a biocontrol agent against soil-borne pathogen Fusarium sambucinum

B. Pérez Bibbins | C. Ghidelli

32. Initial results of WGBS to unveil the role of cytosine methylation promoted by the biocontrol agent P. mediterranea in Citrus limon affected by Mal secco disease

33. Functional characterization of Dormancy-Associated MADS-Box genes in apple

34. Improving drought resistance in strawberry by biotechnology

35. The molecular regulatory mechanism of strawberry fruit color

36. Uptake of dsRNA by plant pathogens: a study of dynamics and efficiency

K. Kostov | G. Smagghe

37. RNAi in pest insects important in horticulture: what do we know, and what do we not know yet?

G. Smagghe

38. Spray-induced gene silencing for improving chestnut tolerance to pathogens

39. Non transformative RNA interference protocols for gene silencing in the Japanese beetle Popillia japonica

40. Environmental stability and degradation of RNA in horticultural production systems – a genetic ecology perspective

M.H. Hagemann

41. Characterization of extracellular vesicles in fall armyworm cells as potential transport of RNA: first data

G. Smagghe | Jialing Liu | Yuqian Cheng | Dongdong Liu

42. Isolation of extracellular vesicles and characterization of their role as shuttle of biologically active small RNAs in intercellular communication

T. Sanson | M. Cecchin | B. Molesini | T. Pandolfini

43. RNAi of ecdysteroid-related genes inhibits the molting process of spider mites (Tetranychus urticae)

G. Smagghe | Zhou Li | Gang Li

44. Non transformative plant-mediated delivery of dsRNAs targeting the leafhopper Scaphoideus titanus, vector of grapevine flavescence dorée phytoplasmas

F. Canuto | C. Marzachì | D. Bosco | L. Galetto

45. Establishment of in vitro regeneration protocols and protoplasts isolation techniques for Sicilian grapevine cultivars

46. Flower bud morphology as an indicator of microspore/pollen development in Vicia faba

I. Moshood | W. Skrzypkowski | J.M. Seguí-Simarro | A. Kiełkowska

47. Enhancing Vaccinium corymbosum regeneration by genome engineering

48. Optimized protoplast technology for highbush blueberry: a gateway to precision breeding

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49. Indirect plant regeneration of drought-resistant ecotypes of Lavandula latifolia Medik.

G. Cáceres-Cevallos | T. Lopes | M. Jordán | S. Correia

50. Moracin M production from root cultures in mulberry (Morus alba L.)

51. De novo regeneration capacity of different explants of Japanese plum (Prunus salicina Lindl.)

52. An improved regeneration protocol for the establishment of in vitro cultures of Juniperus phoenicea L.

K. Bertsouklis | P. Aroniada | P. Stamatopoulou | A. Paraskevopoulou

53. Effect of pretreatment media on adventitious shoot regeneration from leaves of plum (Prunus domestica ‘Sirma’)

L. Nacheva | N. Dimitrova | D. Aleksandrova | N. Keranova

54. Optimization of protoplast isolation and culture protocol for Mediterranean-adapted broccoli (Brassica oleracea var. italica) cultivars

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55. Mutant breeding of crape myrtle (Lagerstroemia L.)

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56. Protoplast technology and somatic hybridization in strawberry

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57. Comparison of ovary culture and irradiated pollen technique used for haploid plant production in cucumber

58. Genome-wide association studies reveal a key region in chromosome 2 to explain variation in dehydroascorbic acid content in lettuce (Lactuca sativa L.)

59. Molecular based cultivar development by using ovary culture method and cytoplasmic genome prediction in cucumber

60. Physiological and transcriptomic insights into drought tolerance in eggplant (Solanum melongena L.)

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63. Revisiting transgenic hops for sustainable resveratrol production and potential applications in brewing and pharmacology

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64. A molecular phenology scale of grape berry development

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67. Biosafety regulation of biotech crops in Ecuador

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69. Insights into population structure and genetic diversity in lettuce (Lactuca sativa L.) based on SNP genotyping data

I. Medina-Lozano | J. Plieske | M. Ganal | H. Gnad | A. Díaz

70. Molecular insights into water stress responses in the ‘Italia’ table grape cultivar

71. Differentiation of grape cultivars (Vitis vinifera L.) from the Peloponnese based on the ampelographic description and molecular method SSR

I. Daskalakis | M. Stavrakaki | D. Bouza | D. Rusjan | K. Biniari

72. Application of SNP molecular markers for bitterness trait in nineteen parental lines of parthenocarpic cucumber

P. Laklai | N. Nitwatthakul | A. Tira-umphon

73. Generation of a semidiallelic cross in Vitis vinifera for enhanced genomic selection

74. Evaluation of strawberry runner production in controlled environment agriculture systems

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75. DNA barcode as a tool for molecular identification of plants

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