Articles
Flower bud morphology as an indicator of microspore/pollen development in Vicia faba
Article number
1454_46
Pages
327 – 334
Language
English
Abstract
The development of an efficient protocol for in vitro induction of androgenesis is pivotal for applying biotechnological approaches to aid breeding of Vicia faba L. This species remains highly recalcitrant to induction of microspore embryogenesis.
One of the main factors determining the success of induction of microspore embryogenesis is the use of the most responsive stages during microspore/pollen development (mainly microspores and in some species young bicellular pollens). For this, it is essential to find accurate and convenient morphological markers to identify flower buds containing these stages.
Flower bud morphology, bud size and anther color are well-defined and useful markers in other species, but their application in V. faba remains underexplored.
This study examined the relationship between flower bud morphology and pollen developmental stages in two cultivars, ‘Karmazyn’ and ‘Bonus,’ through cytological analyses.
Results showed asynchronous pollen development within anthers, with multiple microspore/pollen developmental stages coexisting in the same anther.
Measurements revealed cultivar-specific correlations between bud dimensions and critical stages for androgenesis induction.
Both cultivars exhibited overlapping developmental stages within individual anthers, complicating precise identification.
Preliminary findings suggest that focusing on specific bud length and width ranges could serve as a reliable morphological marker for selecting buds for androgenesis.
This approach addresses challenges posed by variations in flower morphology and intracultivar differences.
Future studies will refine these criteria to improve protocols, therefore contributing to improved plant breeding in V. faba.
One of the main factors determining the success of induction of microspore embryogenesis is the use of the most responsive stages during microspore/pollen development (mainly microspores and in some species young bicellular pollens). For this, it is essential to find accurate and convenient morphological markers to identify flower buds containing these stages.
Flower bud morphology, bud size and anther color are well-defined and useful markers in other species, but their application in V. faba remains underexplored.
This study examined the relationship between flower bud morphology and pollen developmental stages in two cultivars, ‘Karmazyn’ and ‘Bonus,’ through cytological analyses.
Results showed asynchronous pollen development within anthers, with multiple microspore/pollen developmental stages coexisting in the same anther.
Measurements revealed cultivar-specific correlations between bud dimensions and critical stages for androgenesis induction.
Both cultivars exhibited overlapping developmental stages within individual anthers, complicating precise identification.
Preliminary findings suggest that focusing on specific bud length and width ranges could serve as a reliable morphological marker for selecting buds for androgenesis.
This approach addresses challenges posed by variations in flower morphology and intracultivar differences.
Future studies will refine these criteria to improve protocols, therefore contributing to improved plant breeding in V. faba.
Authors
I. Moshood, W. Skrzypkowski, J.M. Seguí-Simarro, A. Kiełkowska
Keywords
microsporogenesis, androgenesis, morphological markers, legumes, haploidization, doubled haploids
Groups involved
- Division Plant Genetic Resources, Breeding and Biotechnology
- Working Group Genetic Transformation and Gene Editing
- Working Group Horticultural Biotechnology and Breeding
- Division Ornamental Plants
- Division Vegetables, Roots and Tubers
- Division Temperate Tree Fruits
- Division Vine and Berry Fruits
- Division Horticulture for Development
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