Home > IV International Symposium on Flower Bulbs > PROBLEMS CONCERNING THE PRODUCTION OF MONOCLONAL ANTIBODIES FOR PLANT DIAGNOSTIC PURPOSES

Articles

PROBLEMS CONCERNING THE PRODUCTION OF MONOCLONAL ANTIBODIES FOR PLANT DIAGNOSTIC PURPOSES

Article number

177_13

Pages

103 – 110

Language

Abstract

Although the potential diagnostic power of monoclonal antibodies (MCAs)* is superior to polyclonal antisera, a number of problems have to be solved before MCAs may be applied for diagnostic purposes in plants.
The data on MCAs against plant growth regulators and plant viruses show that these problems include the immunization protocol, ELISA procedures during cell cloning, stability of clones and the production of ascites.
In addition application may be hampered since MCAs may be too specific and lose activity after coating of ELISA plates or after conjugation.

Publication

IV International Symposium on Flower Bulbs

Authors

P.M. Boonekamp, H. Pomp

Keywords

Full text

https://doi.org/10.17660/ActaHortic.1986.177.13

Online Articles (125)

1. INNOVATION: A CHALLENGE TO FLOWER-BULB INDUSTRY AND RESEARCH

Robert J. Bogers

2. QUALITY CONTROL – INCENTIVES TO IMPROVEMENT

Ir. H. van Os

3. FUNGICIDE-RESISTANCE: HOW DOES IT ARISE AND WHAT CAN WE DO ABOUT IT?

J. Dekker

4. POSSIBILITIES AND PROSPECTS FOR THE DEVELOPMENT OF BIOLOGICAL AND OTHER ALTERNATIVE METHODS OF DISEASE CONTROL IN BULBS AND CORMS

J.R. Coley-Smith

5. NARCISSUS SMOULDER (SCLEROTINIA NARCISSICOLA GREG.) – A DISEASE RELATED TO HOST INJURY

G.R. Dixon

6. THE SUSCEPTIBILITY OF NARCISSUS SPECIES TO INFECTION BY FUSARIUM OXYSPORUM F. SP. NARCISSI

C. A. Linfield

7. SCREENING BULBILS, CHIPS, TWIN SCALES AND SEEDLINGS OF SEVERAL CULTIVARS FOR RESISTANCE TO FUSARIUM OXYSPORUM F.SP. NARCISSI

C. A. Linfield | D. Price

8. NARCISSUS VARIETAL SUSCEPTIBILITY TO FUSARIUM OXYSPORUM (BASAL ROT)

A.A. Tompsett

9. SOME ASPECTS OF THE EPIDEMIOLOGY OF FUSARIUM OXYSPORUM F.SP. GLADIOLI ON FREESIA

H. Rattink

10. HUSBANDRY PRACTICES AND THE CONTROL OF PENICILLIUM CORYMBIFERUM ON CROCUS

M.W. Sutton | S.J. Wale

11. LEAF CURLING OF ANEMONE: SURVIVAL OF THE PATHOGEN AND CONTROL OF THE DISEASE (*)

M. Lodovica Gullino | G. Lento | A. Garibaldi

12. COLLETOTRICHUM GLOEOSPORIOIDES ON ANEMONE IN FRANCE

A. Tramier | A. Bertaccini

13. PROBLEMS CONCERNING THE PRODUCTION OF MONOCLONAL ANTIBODIES FOR PLANT DIAGNOSTIC PURPOSES

P.M. Boonekamp | H. Pomp

14. THE POSSIBLE APPLICATION OF MONOCLONAL ANTIBODIES IN ELISA FOR DETECTION OF TULIP BREAKING VIRUS

J.M. Franssen | C.Th.C. van der Hulst

15. DETECTION OF BACTERIA AND VIRUS IN DAHLIA BY IMMUNO-ENZYMATIC TESTS

D. Goumas | M. Lemattre | J.P. Narcy | J. Albouy | N. Larroque

16. DETECTION OF TULIP BREAKING VIRUS AND LILY SYMPTOMLESS VIRUS IN LILY BULBS BY MEANS OF ELISA.

A.R. van Schadewijk

17. THE EFFECT OF GIBBERELLIC ACID AND ABSCISIC ACID ON TULIP PISTIL GROWTH IN VITRO

L. Kawa | M. Saniewski

18. DIFFERENTIAL ACTION OF IAA AND NAA, DEPENDENT ON THE TRANSPORT DIRECTION, ON PERIANTH AND PISTIL GROWTH IN TULIPS

M. Saniewski

19. EFFECTS OF DURATION OF EXPOSURE TO ETHYLENE ON FLOWERING OF DUTCH IRIS

H. Imanishi | D. Yue

20. EFFECT OF SHORT PHOTOPERIOD AND GROWTH REGULATORS ON GROWTH, FLOWERING AND TUBERIZATION OF BEGONIA X TUBERHYBRIDA

J. Tonecki

21. FLOWER FORMATION IN EUCHARIS AMAZONICA LINDEN EX PLANCHON

J. van Bragt | W. Luiten | P.A. Sprenkels | C.J. Keijzer

22. GROWTH AND FLOWERING OF RANUNCULUS ASIATICUS

K. Ohkawa

23. EFFECTS OF ROOT- AND AERIAL PART SUPPRESSION IN THE PLANT ON FLOWER INITIATION IN THE TULIP BULB

M. Le Nard

24. THE INFLUENCE OF 32°, 21°, 4° OR 27°, 18°, 10° OR 21°, 17°, 13°C LIGHT/DARK TEMPERATURE COMBINATIONS DURING THE VARIOUS GROWTH PHASES OF THE EASTER LILY (LILIUM LONGIFLORUM THUNB.)

H.F. Wilkins

25. RETARDING THE FLOWERING OF NARCISSUS TAZETTA CV. ‘ZIVA’

H. Yahel | D. Sandler

26. EFFECT OF VARIOUS CORM TREATMENTS ON FLOWERING OF LIATRIS SPICATA WILLD.

R. Moe | M. Berland

27. STARCH METABOLISM IN FLOWERS OF SENESCING GLADIOLI INFLORESCENCES

D.I. Ferreira | J.J. van der Merwe | G.H. de Swardt

28. METHODS OF VIRUS-INDEXING FOR VIRUS-TESTED NARCISSUS STOCKS

W.P. Mowat

29. VIRUS-TESTED NARCISSUS: PROGRESS WITH FIELD EVALUATION IN SCOTLAND

M.W. Sutton | G.R. Dixon | M. Willock

30. FOUR VIRUSES OF ALSTROEMERIA IN BRITAIN

S. Phillips | A.A. Brunt

31. INVESTIGATING RESISTANCE IN TULIP TO TULIP BREAKING VIRUS AND TO ITS TRANSMISSION

Louise R. Romanow | J.P. van Eijk | W. Eikelboom

32. VIRUS DISEASES IN GLADIOLUS AND THEIR CONTROL IN THE NETHERLANDS

C.J. Asjes

33. MINIMUM FLUORESCENT LIGHT REQUIREMENTS AND ANCYMIDOL INTERACTIONS ON THE GROWTH OF LILIUM LONGIFORUM THUNB. ‘NELLIE WHITE’

K. Grueber | W. Healy | H.B. Pemberton | H.F. Wilkins

34. EFFECT OF NITROGEN ON BULB PRODUCTION, FORCING QUALITY OF THE BULB AND VASE LIFE OF THE FLOWER OF LILIUM ‘ENCHANTMENT’

J. van der Boon | H. Niers

35. FLOWER BULB FORCING IN KENYA

K. Waithaka

36. NARCISSUS: FLOWERS PER TONNE OF BULBS

A.R. Rees

37. COMMERCIAL FORCING AND MARKETING REQUIREMENTS FOR FLOWER-BULBS IN THE U.S. AND CANADA

A.A. De Hertogh | M. Kamp

38. ANALYSIS OF DIFFERENCES IN PHYSICAL AND FINANCIAL CROP RESULTS OF FORCED TULIP FLOWERS

C.O.N. de Vroomen

39. IN VITRO PROPAGATION OF BULBOUS CROPS

J. van Aartrijk | P.C.C. van der Linde

40. MULTIPLICATION OF TULIP BULB BY TISSUE CULTURE IN VITRO

Y. Nishiuchi

41. ARE WE ANY NEARER COMMERCIAL MICROPROPAGATION OF TULIPS?

A.D. Paterson | P.C. Harper

42. MICROPROPAGATION OF TULIP: BULBING OF SHOOTS IN CULTURE

P.G. Alderson | A.G. Taeb | R.D. Rice

43. BYMV-FREE CLONES OF EIGHT GLADIOLUS CULTIVARS OBTAINED BY MERISTEM-TIP CULTURE

A. Bertaccini | F. Marani

44. NARCISSUS BULB MORPHOLOGY AND TWIN-SCALE PROPAGATION

Gordon R. Hanks

45. NARCISSUS PROPAGATION BY CHIPPING: EFFECT OF A RANGE OF PLANT GROWTH REGULATORS ON BULBIL YIELD AND LENGTH

G.J. Flint | P.G. Alderson

46. FACTORS AFFECTING THE INDUCTION OF CONTRACTILE ROOTS IN GLADIOLUS

A. H. Halevy

47. EFFECTS OF LIGHT ON THE TIME OF FLOWERING, YIELD AND QUALITY OF CORM FREESIAS GROWN IN GLASSHOUSES

J.C. Doorduin | C.M.M. van Winden

F.T. Heidema | G. Grevers | N. Gorin | C.T.C.v. Hulst | J.M. Franssen

49. LOW TEMPERATURE EFFECTS ON CALADIUM TUBERS

A. Borochov | A. Lavee | A.H. Halevy

50. PRODUCTION OF GLORIOSA TUBERS FROM SEEDS

J. Krause

51. THE EFFECTS OF PROTECTED CULTIVATION, PRE-SPROUTING AND LATERAL TUBER REMOVAL ON TUBEROSE PRODUCTION

David J. Brundell | Don R. Steenstra

52. STUDIES ON THE CORM PRODUCTION OF GLADIOLUS IN THE SUBTROPICAL REGION OF PUNJAB, INDIA

A.P.S. GILL | M. AULAKH | L. PASWAN

53. THE IDENTIFICATION OF ANTHOCYANIN PIGMENTS IN THE PETALS AS AN AID TO THE BREEDING OF GLADIOLUS

A. Cohen | N. Akavia | N. Umiel

54. IMPROVING FORCING ABILITY – THE BREEDER’S CONTRIBUTION

L.D. Sparnaaij | J.P. van Eijk | L. Smeets

55. BREEDING FOR EARLY FLOWERING OF ASIATIC LILIES

K. Mynett

56. THE IMPORTANCE OF WILD SPECIES AND OLD CULTIVARS FOR THE BREEDING OF FLOWERBULBS

J.P. van Eijk | W. Eikelboom | N.G. Hogenboom

57. INDIGENOUS FLOWER BULBS OF SOUTH AFRICA A SOURCE OF NEW GENERA AND SPECIES FOR ORNAMENTAL BULB CULTIVATION

D.I. Ferreira | F.L. Hancke

58. TULIPS AS A ONE AND TWO YEAR CULTURE IN DENMARK

E. Rasmussen

59. BULBWEIGHT PARTITIONING PATTERNS OF SOME TULIP CULTIVARS

G.G.M. van der Valk | P.N.A. Bruin

60. INFLUENCE OF MOTHER BULB NITROGEN ON SUBSEQUENT NITROGEN UPTAKE IN TULIPS

M. Amano

61. EFFECT OF CALCIUM ON TIPBURN OF LILIUM ‘PIRATE’

J. Berghoef

62. OBSERVATIONS ON THE DEVELOPMENT AND CULTURE OF SANDERSONIA

David J. Brundell | Jenny L. Reyngoud

63. STUDY ON TULIP FIRE CAUSED BY BOTRYTIS TULIPAE (LIB.) LIND IN BULGARIA

R. Bachvarova | S. Dimitrov | I. Filipova

64. CONTROL OF FIRE (BOTRYTIS TULIPAE) ON TULIPS IN WASHINGTON WITH DICARBOXIMIDE FUNGICIDES

Gary A. Chastagner

65. IN VITRO PRODUCTION OF BOTRYOTINIA POLYBLASTIS APOTHECIA AND FACTORS AFFECTING GERMINATION OF ASCOSPORES

Gary A. Chastagner

66. FUSARIUM WILT OF CYCLAMEN IN SPAIN

F.J. Tello | L. Vares | F. Vares | A. Mijares

67. THE BEHAVIOUR OF VARIOUS PRODUCTS IN CONTROLLING FUSARIUM OXYSPORUM IN IXIA SPP.

M.G. Fantino | M.R. Contarini | C. Pasini

68. INVESTIGATION OF FUNGICIDE TREATMENTS TO CONTROL ANEMONE LEAF CURL DISEASE CAUSED BY A SPECIES OF COLLETOTRICHUM

T.M. O'Neill

69. INDICATIONS FOR TRANSMISSION OF COLLETOTRICHUM ACUTATUM VIA ROOTS OF ANEMONE CORONARIA

A.W. Doornik

70. BASAL PLATE ROT OF NARCISSUS BULBS AND ITS CONTROL

H. Vigodsky-Haas | A. Lavi

71. LEAF-TIP NECROSIS IN FORCED TULIPS AS A RESULT OF A ROOT DECAY CAUSED BY TRICHODERMA VIRIDE

P.J. Muller

72. CHEMICAL CONTROL OF PENICILLIUM SP. ON TULIP BULBS USED FOR FORCING

L. Ondrušková

73. EFFECT OF FOLIAR FUNGICIDES ON CONTROL OF BOTRYTIS TULIPAE /LIB./ LIND. ON TULIPS

Cz. Skrzypczak | L. Orlikowski

74. GLADIOLUS ROT CONTROL EXPERIENCE

Zlata K. Šmit | M. Ševar

75. SOME FACTORS AFFECTING THE INCIDENCE OF BACTERIAL SOFT ROT IN BULBOUS IRIS

W. Kamerman

76. CONTROL OF VOLUNTEER CEREALS AFTER EMERGENCE OF BULBOUS CROPS

A.Th.J. Koster | L.J.v.d. Meer

77. CONTROL OF ACYLALANINE-RESISTANT PYTHIUM SPP. DURING FORCING OF TULIPS IN GLASSHOUSES

A.Th.J. Koster | P. Vink | L.J. van der Meer

78. CONTROL OF SOIL-BORNE DISEASES, NEMATODES AND WEEDS IN ORNAMENTAL BULB CULTIVATION BY MEANS OF FLOODING

79. CONTROL OF TAENIOTHRIPS SIMPLEX MORISON ON GLADIOLUS CORMS IN STORAGE ROOMS

C.G.M. Conijn | A.Th.J. Koster | P.J. Muller

80. FOAMING, A NEW METHOD FOR PESTICIDE APPLICATION IN BULB GROWING.

R.S. Bijl

81. MYCOLOGICAL PROSPECTION OF THE GLADIOLUS CORM IMPORTS IN SPAIN

J. Garcia-Jimenez | V.J. Piera | A. Alfaro

82. AN IMPROVED METHOD TO EVALUATE FUSARIUM OXYSPORUM F.SP. GLADIOLI PATHOGENICITY

J. Garcia-Jimenez | V.J. Piera | A. Alfaro

83. GLADIOLUS VIRUSES ISOLATED FROM GLADIOLUS BULBS (GLADIOLUS SPP.) IN ITALY

M.G. Bellardi | A. Pisi

84. RATES OF REINFECTION WITH VIRUS IN COMMERCIAL GLADIOLUS CROPS

Jane R. Moran | Carol E. Curtis

A.F.L.M. Derks | Th.C. Hollinger

86. THE DEVELOPMENT OF ELISA TO DETERMINE ABSCISIC ACID AND INDOLE-3-ACETIC ACID IN BULBOUS CROPS

J.M. Franssen | B.E. Snaar-Jagalska | C.Th.C. van der Hulst

87. TOWARDS “IN VITRO” PROPAGATION OF BULBOUS IRIS

P.C.G. van der Linde | G.J. Blom-Barnhoorn | J. van Aartrijk

88. EFFECTS OF INDOLE BUTYRIC ACID AND BENZYLADENINE ON THE PROCESS OF ADVENTITIOUS PLANTLET FORMATION “IN VITRO” ON BULB-SCALE EXPLANTS OF HYACINTH

J. van Aartrijk | G.J. Blom-Barnhoorn | P.C.C. van der Linde

89. THE EFFECT OF A PRETREATMENT AT THE GROWERS ON THE BULB FLOWER QUALITY

A. Swart

90. EFFECT OF VIRAZOLE ON THE PRODUCTION OF HYACINTH PLANTS FREE FROM HYACINTH MOSAIC VIRUS (HMV) BY MERISTEM CULTURE

Gerry J. Blom-Barnhoorn | J. van Aartrijk | P.C.G. van der Linde

91. DISEASE INDEXING OF ORNAMENTAL BULB CROPS AND LARGE-SCALE PRODUCTION OF FIRST GENERATION CLEAN MOTHER STOCK THROUGH TISSUE CULTURE

S.C. Worfolk | R. deLacy

92. EFFECT OF SOME GROWTH REGULATORS ON THE PROPAGATION OF HYACINTHUS ORIENTALIS AND TULIPA GESNERIANA

V. Kapchina | M. Dinova | I. Ivanova

93. THE PRODUCTION OF VIRUS-TESTED NARCISSUS IN SCOTLAND

W.P. Mowat | G.R. Dixon | W. Fordyce

94. NARCISSUS CERTIFICATION IN SCOTLAND

P.A. Rankin

95. CHIPPING OF NARCISSUS BULBS: A QUICK WAY TO OBTAIN LARGE NUMBERS OF SMALL, ROUND BULBS.

P.J.M. Vreeburg

96. BREEDING FOR RESISTANCE TO YELLOW DISEASE OF HYACINTHS

Jaap M. van Tuyl | Joop P. van Eijk | Ton A.M. Kwakkenbos

97. INTERSPECIFIC CROSSES IN TULIP BREEDING

J.P. van Eijk | W. Eikelboom | J.B.M. Custers | J. Franken | L.W.D. van Raamsdonk

98. INTERSPECIFIC HYBRIDIZATION IN LILIUM

Jaap M. van Tuyl | J. Franken | Ria C. Jongerius | C. A.M. Lock | Ton A.M. Kwakkenbos

99. PERFORMANCE TRIALS ON VARIETIES OF BULBS

H.P. Pasterkamp

100. SELECTION FOR FUSARIUM RESISTANCE IN NERINE BOWDENII

J.M. van Tuyl | C.A.M. Lock | A.C. van der Giessen

101. IDENTIFICATION OF INTERSPECIFIC HYBRIDS AND DETERMINATION OF RELATIONSHIPS BETWEEN SPECIES IN THE GENUS LILIUM BY ISOELECTRIC FOCUSING

J.M. van Tuyl | A.J. van Dijk | L.W.D. van Raamsdonk

102. THE TOLERANCE OF TULIP PLANT MATERIAL TO HIGH CO₂ AND LOW O₂ CONCENTRATIONS IN RELATION TO VENTILATION AND AIR CIRCULATION IN BULB STORAGE ROOMS

W.J. de Munk | T.L.J. Duineveld

103. EFFECT OF LOW LIGHT INTENSITY ON FLOWERING OF ASIATIC LILIES

Jaap M. van Tuyl | Ton A.M. Kwakkenbos

104. GROWTH REGULATORS AND γ – IRRADIATION TREATMENTS AFFECTING THE SPROUTING AND ENDOGENOUS HORMONES IN GLADIOLUS

A. R. E. Awad | K. Elbahr

105. THE EFFECT OF PHOTOPERIOD AND TEMPERATURE ON THE DEVELOPMENT OF DUTCH IRIS FLOWERS AND DAUGHTER BULBS

E.D. Elphinstone | A.R. Rees | J.G. Atherton

106. THE INFLUENCE OF ETHYLENE, HIGH TEMPERATURES AND ANAEROBIOSIS ON CYANIDE-RESISTANT RESPIRATION AND FLOWERING OF IRIS BULBS.

A. Marissen | L.H.W. van der Plas

107. STIMULATION AND SUPPRESSION OF FLOWER FORMATION AND DEVELOPMENT IN IRIS BY CASEOUS COMPOUNDS

T.L.J. Duineveld | W.J. de Munk

108. EFFECTS OF LIGHT DURING SPECIAL PRECOOLING OF TULIP BULBS CV. PAUL RICHTER ON THE CONTENT OF CHLOROPHYLLS IN THE SHOOTS AND ON SUBSEQUENT ANTHESIS

N. Gorin | H. Zonneveld

109. THE EFFECT OF TEMPERATURE AND ETHYLENE ON DORMANCY OF FREESIA CORMS

J. Berghoef | A.P. Zevenbergen | H. Imanishi

110. EFFECT OF TEMPERATURE ON INITIATION AND DEVELOPMENT OF FREESIA FLOWERS

J. Berghoef | J.W.F. Melcherts | J.A.M. Mourits | A.P. Zevenbergen

111. SOME FACTORS AFFECTING DORMANCY-BREAKING BY ETHYLENE IN FREESIA CORMS

H. Imanishi | J. Berghoef

112. ON THE ROLE OF ETHYLENE BIOSYNTHESIS IN FLOWER-BUD ABSCISSION OF LILIUM X ‘ENCHANTMENT’

U. van Meeteren | G. Slootweg

113. POSSIBILITIES TO FORCE MINIATURE GLADIOLUS YEAR ROUND?

U. van Meeteren | J. Berghoef | G. Slootweg | A.P. Zevenbergen

114. FLOWERING OF GLADIOLUS CV. ROSELIND IN APRIL AND OCTOBER

N.P.A. Groen

115. FLOWERING IN IXIA: INFLUENCE OF STORAGE TEMPERATURE AND PLANTING DATE UNDER NATURAL AND ARTIFICIAL LIGHTING

J.A.Th. de Winter

116. POLISH CULTIVARS OF TULIPS FOR FORCING

M. Jerzy | M. Zalewska

117. PRELIMINARY EXAMINATION OF SOME FACTORS THAT INFLUENCE THE VASE LIFE OF CUT BULB FLOWERS

R.P. Doss

118. EFFECT OF ETHEPHON AND SILVER ON SOME PROCESSES IN AGEING CUT DAHLIAS

A.J. Lukaszewska

119. LILIUM ZAIRII – A NEW LILIUM FORMOSANUM TYPE FOUND IN ZAIRE

K. Mynett | H. Mackiewicz

120. A RESEARCH ON THE POSSIBILITY OF CONTINUOUS TULIP PRODUCTION IN THE WINTER-SPRING PERIOD.

M. Talia

121. THE GERMINATION OF ANEMONE ‘ST. PIRAN’ SEED AND CORMS

S.K. Jones

122. POT DAFFODILS

F.Th. de Greef

123. NITROGEN UPTAKE BY TULIPS

J. van der Boon

124. YIELD PREDICTION OF TULIP CV. APELDOORN

M. Benschop

125. EPILOGUE

Robert J. Bogers