Articles
ROOTING OF LAPAGERIA ROSEA SHOOTS MULTIPLIED IN VITRO
Article number
226_6
Pages
73 – 80
Language
Abstract
Propagation of named cultivars of Lapageria by cuttings or by layering is slow and micropropagation provides a feasible alternative.
Cultures of the cultivar Nashcourt, multiplied on a Murashige and Skoog medium (M-S) containing l μM BAP, were used in these experiments.
Growth habits in vitro reflected those in vivo.
Lapageria in vivo consisted of an underground rhizome, built up sympodially of successive rhizome buds which eventually grew upwards to form aerial shoots.
Buds developing on aerial shoots grew differently in their initial stages and unlike rhizome buds did not develop roots.
Lapageria proliferated as aerial shoots in vitro but it was also possible to induce the formation of rhizome buds if the development of axillary buds was stimulated in the presence of paclobutrazol.
Such buds contained root primordia and, when transferred to a rooting medium, rooted in 30 days.
Aerial shoots in culture, which contain a developing axillary bud (1–2 cm), also rooted but they took longer.
Both types of shoot rooted in the region of the stem containing the initial few internodes and not on the parent stem.
This paper stresses the importance of understanding how the plant develops in vivo before its behaviour in vitro can be understood.
Cultures of the cultivar Nashcourt, multiplied on a Murashige and Skoog medium (M-S) containing l μM BAP, were used in these experiments.
Growth habits in vitro reflected those in vivo.
Lapageria in vivo consisted of an underground rhizome, built up sympodially of successive rhizome buds which eventually grew upwards to form aerial shoots.
Buds developing on aerial shoots grew differently in their initial stages and unlike rhizome buds did not develop roots.
Lapageria proliferated as aerial shoots in vitro but it was also possible to induce the formation of rhizome buds if the development of axillary buds was stimulated in the presence of paclobutrazol.
Such buds contained root primordia and, when transferred to a rooting medium, rooted in 30 days.
Aerial shoots in culture, which contain a developing axillary bud (1–2 cm), also rooted but they took longer.
Both types of shoot rooted in the region of the stem containing the initial few internodes and not on the parent stem.
This paper stresses the importance of understanding how the plant develops in vivo before its behaviour in vitro can be understood.
Authors
J. McKinless, P.G. Alderson, R.D. Rice
Keywords
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