Articles
EFFECT OF PEG INDUCED OSMOTIC STRESS ON PROLINE, PROTEIN AND RELATIVE WATER CONTENT IN IN VITRO PLANTS OF MACROTYLOMA UNIFLORUM (LAM.) VERDC.
Article number
865_10
Pages
87 – 93
Language
English
Abstract
In vitro plantlets of Macrotyloma uniflorum were obtained on L2 IBA+BAP under water stress conditions induced by supplementing the medium with PEG at various concentrations ranging from 5% to 30% (w/v). Soluble proteins, amino acids, proline, water potential and relative water content were estimated in both control and stress induced in vitro shoots.
Induced water stress has decreased the relative water content and water potential.
However, accumulation of proteins, amino acids and proline was high in in vitro shoots raised on the medium supplemented with PEG at 25%. The in vitro shoots adapted to low concentrations of PEG showed more growth when compared to the plants adapted to high concentration of PEG. Importance of stress induced in vitro plants of Macrotyloma uniflorum in gene transfer technology to raise stress tolerance plants is discussed.
Induced water stress has decreased the relative water content and water potential.
However, accumulation of proteins, amino acids and proline was high in in vitro shoots raised on the medium supplemented with PEG at 25%. The in vitro shoots adapted to low concentrations of PEG showed more growth when compared to the plants adapted to high concentration of PEG. Importance of stress induced in vitro plants of Macrotyloma uniflorum in gene transfer technology to raise stress tolerance plants is discussed.
Publication
Authors
D.H. Tejavathi, V.R. Devaraj, S.M. Murthy, R. Nijagunaiah, K. Shobha
Keywords
Macrotyloma uniflorum, horse gram, induced drought, transgenic plants
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