Home > IV International Symposium on Acclimatization and Establishment of Micropropagated Plants > INDUCTION OF CALLUS FROM HYPOCOTYLEDONS AND COTYLEDONARY LEAVES OF CERATONIA SILIQUA

Articles

INDUCTION OF CALLUS FROM HYPOCOTYLEDONS AND COTYLEDONARY LEAVES OF CERATONIA SILIQUA

Article number

865_39

Pages

293 – 296

Language

English

Abstract

Pale yellow callus was formed from segments of seedlings on MS medium + 2,4-D (3 mg.L^-1) + BAP (3 mg.L^-1) after 4 weeks by 58.3%. No callus was induced in case of using MS medium + NAA (0.5 mg.L^-1) + Kin (1 mg.L^-1) and MS hormone-free.
On the other hand, callus was formed on MS medium+ BAP (2 mg.L^-1) by 71.4%. A large amount of callus formed on MS medium + TDZ (0.5 mg.L^-1) and the callus regenerated small leaflets structures when subcultured on MS + TDZ (1 mg.L^-1) after 4 weeks.
For cotyledonary leaves, callus was formed on MS + NAA (0.5 mg.L^-1) + Kin (1 mg.L^-1) and MS + 2,4 D (3 mg.L^-1) + BAP (3 mg.L^-1) by 25 and 100%, respectively.
No callus was induced in case of using MS hormone-free.
Different concentrations of TDZ were also applied, for increasing and multiplication of the callus from cotyledonry leaves.

Publication

IV International Symposium on Acclimatization and Establishment of Micropropagated Plants

Authors

A.I.M. Saad, G.E. Elnour

Keywords

Full text

https://doi.org/10.17660/ActaHortic.2010.865.39

Online Articles (60)

1. PHOTOAUTOTROPHIC MICROPROPAGATION SYSTEMS – APPLICATIONS TO CONSERVATION BIOTECHNOLOGY PROGRAMMES

V. Sarasan

2. SILICON EFFECTS ON GROWTH ACCLIMATIZATION AND STRESS TOLERANCE OF BIOREACTOR CULTURED ORNITHOGALUM DUBIUM PLANTS

M. Ziv

3. OPTIMIZATION OF PROTOCOLS FOR THE IN VITRO MULTIPLICATION AND CONSERVATION OF ACORUS CALAMUS, AN ENDANGERED MEDICINAL PLANT

P.E. Rajasekharan | B.S. Ravish | T. Vasantha Kumar

4. BERRY BIOREACTOR MICROPROPATION AND MOLECULAR ANALYSIS FOR GENETIC FIDELITY

S.C. Debnath

5. OPTIMIZATION OF PROTOCOLS FOR IN VITRO MULTIPLICATION AND CONSERVATION OF NOTHAPODYTES NIMMONIANA, AN ENDANGERED MEDICINAL PLANT

P.E. Rajasekharan | V.K. Abdul Kareem | T. Vasantha Kumar

6. IN VITRO CULTURE FOR MUTANT DEVELOPMENT

S.M. Jain | S.J. Ochatt | V.M. Kulkarni | S. Predieri

7. USE OF MOLECULAR MARKERS IN CROP IMPROVEMENT OF PUNICA GRANATUM L. FOR WATER STRESS TOLERANCE

N. Adivapp | S.H. Jalikop | T.V. Ananthanarayanan | N.K. Srinivas Rao | R.H. Laxman

8. MOLECULAR ANALYSIS OF MICROPROPAGATED APPLE ROOTSTOCK MM111 USING ISSR MARKERS FOR ASCERTAINING CLONAL FIDELITY

H. Pathak | V. Dhawan

9. STUDY OF THE EXPLANTS FROM TISSUE CULTURE FOR EVALUATION OF SOMACLONAL VARIATION USING RAPD MARKERS

S. Kianamiri | M. Hassani

10. EFFECT OF PEG INDUCED OSMOTIC STRESS ON PROLINE, PROTEIN AND RELATIVE WATER CONTENT IN IN VITRO PLANTS OF MACROTYLOMA UNIFLORUM (LAM.) VERDC.

D.H. Tejavathi | V.R. Devaraj | S.M. Murthy | R. Nijagunaiah | K. Shobha

11. HYPERHYDRICITY – A BOTTLENECK TO MICROPROPAGATION OF PLANTS

B.N. Hazarika | A. Bora

12. MERITS AND DRAWBACKS OF NEW AROMATIC CYTOKININS IN PLANT TISSUE CULTURE

S.P.O. Werbrouck

13. OPTIMIZATION OF SPILANTHES ACMELLA L. CULTIVATION BY IN VITRO NODAL SEGMENT CULTURE

M. Singh | R. Chaturvedi

14. ESTABLISHMENT OF EFFICIENT REGENERATION SYSTEM, ACCLIMATIZATION AND SOMACLONAL VARIATION IN SAINTPAULIA IONANTHA H. WENDL.

R.M. Taha | N. Daud | N.A. Hasbullah

15. ANATOMY OF ROOT DEVELOPMENT IN TISSUE CULTURE RAISED TERMINALIA BELLERICA ROXB. AND ITS SIGNIFICANCE IN HARDENING

R.K. Suthar | S.D. Purohit

16. ACCLIMATIZATION STUDIES IN STEVIA (STEVIA REBAUDIANA BERT.)

A.V. Meera Manjusha | B.N. Sathyanarayana

17. THE EFFECT OF BENZYL ADENINE (BA) HORMONE AND EXPLANTS TYPE ON ESTABLISHMENT AND PROLIFERATION OF IRANIAN DWARFING APPLE ROOTSTOCK ‘AZAYESH’ UNDER IN VITRO CONDITION

S. Kianamiri | M. Hassani

18. EFFECTS OF HORMONES, EXPLANT AND MEDIA ON ROOTING AND STABILIZATION OF BERMUDAGRASS (CYNODON DACTYLON) IN TISSUE CULTURE

M. Taghizadeh | M. Kafi | R. Fatahi

19. EFFECT OF CO₂ ENRICHMENT ON IN VITRO GROWTH, HARDENING AND ACCLIMATIZATION DURING MICROPROPAGATION OF SOME TREE SPECIES OF ARAVALLIS IN RAJASTHAN

S.D. Purohit | N. Habibi

20. UTILIZING HYDROPONICS TECHNIQUE FOR ACCLIMATIZING TISSUE CULTURE DERIVED PLANTLETS UNDER DESERT ENVIRONMENT

N.S. Al-Khalifah | A.E. Shanavaskhan | F. Ahmed Khan

21. STUDIES ON PRE ACCLIMATIZATION TREATMENTS TO IMPROVE POST ACCLIMATIZATION SURVIVAL IN STEVIA (STEVIA REBAUDIANA BERT.)

A.V. Meera Manjusha | B.N. Sathyanarayana

22. MYCORRHIZA-LIKE STRUCTURES IN ROOTED MICROSHOOTS OF PINUS PINEA L.

23. STOMATAL CHARACTERISTICS DURING MICROPROPAGATION OF WRIGHTIA TOMENTOSA

K. Khan | P. Joshi | S.D. Purohit

24. ROOT COLONIZATION AND IMPROVED GROWTH PERFORMANCE OF MICROPROPAGATED TERMINALIA BELLERICA ROXB. PLANTLETS INOCULATED WITH PIRIFORMOSPORA INDICA DURING EX VITRO ACCLIMATIZATION

M. Chittora | R.K. Suthar | S.D. Purohit

25. FIELD PERFORMANCE, RAPD ANALYSIS AND WITHANOLIDE PROFILING OF MICROPROPAGATED PLANTS OF WSR – AN ELITE CULTIVAR OF INDIAN GINSENG (WITHANIA SOMNIFERA L. DUNAL)

26. EFFECT OF INORGANIC FERTILIZER, MYCORRHIZA AND FARM YARD MANURE ON GROWTH OF MICROPROPAGATED APPLE ROOTSTOCK M.7, FOR THE PRODUCTION OF GRAFTABLE PLANTS IN ONE YEAR

H. Pathak | V. Dhawan

27. BIO-ACCLIMATIZATION OF IN VITRO PROPAGATED BANANA PLANTLETS ‘GRAND NAIN’

S.R. Vasane | A.B. Patil | R.M. Kothari

28. PHOTOSYNTHETIC PERFORMANCE OF SHOOTS OF FERONIA LIMONIA GROWN IN VITRO UNDER CARBON DIOXIDE ENRICHED ENVIRONMENT

P. Joshi | S. Vyas | S.D. Purohit

29. PLANT TISSUE CULTURES UBIQUITOUSLY HARBOR ENDOPHYTIC MICROORGANISMS

P. Thomas

30. VIRUS DIAGNOSIS IN SUCKERS AND TISSUE CULTURES OF BANANA

31. CONTAMINATION CONTROL AND DISEASE MANAGEMENT OF BANANA ‘GRAND NAIN’ PLANTLETS

A.B. Patil | S.R. Vasane | K.K. Moharir | R.M. Kothari

32. MICROPROPAGATION STUDIES ON IMPORTANT FOREST TREES OF HIGH IMPORTANCE: AN EFFORT FOR LARGE-SCALE COMMERCIAL MULTIPLICATION AND THEIR FIELD PLANTATION

S. Sharma | N. Rathi | S. Nautiyal | I.D. Arya

33. A PROTOCOL FOR HIGH FREQUENCY PLANT REGENERATION THROUGH SOMATIC EMBRYOGENESIS IN SWERTIA CHIRATA BUCH.-HAM. EX. C.B. CLARKE

Parveen

34. EASY CLONING AND ACCLIMATIZATION OF REBUTIA MUSCULA

D. Orea Coria | A. Medrano Valverde

35. MEZCAL AGAVE MICROPLANTLETS DEVELOPMENT IN DIFFERENT SUBSTRATES

A. Medrano Valverde | D. Orea Coria | E. Villanueva Nava

36. UNCONVENTIONAL SUBSTRATES FOR IN VITRO ROOTING OF REBUTIA

A. Medrano Valverde | D. Orea Coria

37. EX VITRO ECTOMYCORRHIZAL INOCULATION ENHANCES SURVIVAL AND EARLY GROWTH OF MICROPROPAGATED CLONES OF POPULUS × CANESCENS IN METAL-CONTAMINATED SOILS

K. Bojarczuk | B. Kieliszewska-Rokicka

38. INFLUENCE OF LIGHT QUALITY AND INTENSITY ON ADVENTITIOUS ROOT FORMATION IN MICROSHOOTS OF PINUS PINEA L.

C. Ragonezi | M.R. Castro | K. Klimaszewska | M. Lima | M.A. Zavattieri

39. INDUCTION OF CALLUS FROM HYPOCOTYLEDONS AND COTYLEDONARY LEAVES OF CERATONIA SILIQUA

A.I.M. Saad | G.E. Elnour

40. ACCUMULATION OF HEAVY METALS BY SELECTED WILLOW CLONES

J. Malá | H. Cvrčková | P. Máchová

41. IN VITRO INITIATION OF SEEDS AND FURTHER CORM WEIGHT GAIN IN ZEPHYRA ELEGANS

A.K. Vidal | Y. Niimi

42. DEVELOPMENT OF MICROPROPAGATED SHOOTS AND PLANTS OF CAROÁ IN DIFFERENT SUBSTRATES

D.G. Silveira | J.R.F. Santana | F.V.D. Souza | C.A.S. Ledo | E.C. Cunha

43. MICROPROPAGATION OF HELICONIA ROSTRATA AND HELICONIA BIHAI FROM MATURE ZYGOTIC EMBRYOS

E.H. Souza | T.L. Soares | F.V.D. Souza | J.A. Santos-Serejo

44. INFLUENCE OF THIRTEEN DIFFERENT STRAINS OF ERICOID ENDOMYCORRHIZAE ON ROOTING AND GROWTH OF MICROPROPAGATED AZALEA MOLLIS

T. Eccher | M.C. Piagnani | C. Zordan

45. INOCULATION OF RHODODENDRON CULTIVARS IN VITRO WITH DIFFERENT STRAINS OF ERICOID ENDOMYCORRHIZAE

T. Eccher | M. Martinelli

46. PHOTOAUTOTROPHIC MICROPROPAGATION AND ITS RELEVANCE IN THREATENED PLANT CONSERVATION

P. Marriott | W. Armstrong | J. Armstrong | S. Gorny | V. Sarasan

47. IN VITRO ROOTING AND HARDENING IN APPLE ROOTSTOCK EMLA111 – INFLUENCE OF SOME FACTORS

M. Modgil | R. Gupta | M. Thakur

48. MOLECULAR ANALYSIS OF MICROPROPAGATED STRAWBERRY PLANTS USING ISSR (INTER SIMPLE SEQUENCE REPEATS) MARKERS FOR ASCERTAINING CLONAL FIDELITY

S. Sen | V. Dhawan

49. THE ORIGIN AND DEVELOPMENT OF SECONDARY SOMATIC EMBRYOS IN BANANA CV. NANJANGUD RASBALE (SILK GP. RASTHALI, AAB)

M. Sukhada | K.Y. Prathibha | H.D. Soumya | R.M. Ranganath

50. INVESTIGATION ON TWO METHODS OF IN VITRO MICROPROPAGATION OF EUCALYPTUS MACULATA

M.H. Assareh | M. Sedaghati | K. Kiarostami | A. Ghamari Zare | M.B. Rezaii

51. SYSTEM FOR THE PRODUCTION OF TRANSGENIC BY2 CELLS IN BIOREACTORS

I. Oyanguren | S. Castañón | A. García-Ocaña | R. Vasavada | I. Urreta

52. IN VITRO REGENERATION OF PINUS SPP. ADULT TREES: NEW METHOD FOR OBTAINING CLONAL PLANTS

N. De Diego | I.A. Montalbán | P. Moncaleán

53. MULTIPLE SHOOT REGENERATION FROM THE CULTURES OF ADHATODA VASICA NEES.

D.H. Tejavathi | B.L. Manjula | P. Anitha

54. IN VITRO STUDIES IN SAUROPUS ANDROGYNOUS (L.) MERR.

D.H. Tejavathi | S. Padma | K. Gayathramma | B. Pushpavathi

55. CHEAPER WATER SOURCES FOR MICROPROPAGATION OF BANANA (MUSA ACUMINATE ‘GRANDE NAINE’)

G. Prabhuling | B.N. Sathyanarayana | G. Shivayogeppa | L. Rajan | J. Dinakar Adiga

56. FIRST REPORT ON CALLUS INDUCTION IN ASHOKA (SARACA INDICA): AN IMPORTANT MEDICINAL PLANT

A.A. Waman | K. Umesha | B.N. Sathyanarayana

57. IN VITRO APPROACHES FOR CONSERVATION OF DAHLIA (DAHLIA VARIABILIS L.)

G. Shivayogeppa | J. Dinakar Adiga | G. Prabhuling | B.S. Reddy | B.N. Sathyanarayana

58. STRATEGIES FOR IN VITRO CONSERVATION OF DAHLIA (DAHLIA VARIABILIS L.)

G. Shivayogeppa | J. Dinakar Adiga | G. Prabhuling | B.S. Reddy | B.N. Sathyanarayana

59. ACCLIMATIZATION OF DIANTHUS CARYOPHYLLUS LINN.

A.H. Abdul Aziz | R.M. Taha | N.A. Hasbullah

60. ESTABLISHMENT OF IN VITRO PLANTLETS AND ACCLIMATIZATION OF GERBERA JAMESONII BOLUS EX. HOOK F.

R.M. Taha | N.A. Hasbullah | A.H. Abdul Aziz | A. Awal