Articles
FIELD PERFORMANCE, RAPD ANALYSIS AND WITHANOLIDE PROFILING OF MICROPROPAGATED PLANTS OF WSR – AN ELITE CULTIVAR OF INDIAN GINSENG (WITHANIA SOMNIFERA L. DUNAL)
Article number
865_25
Pages
199 – 207
Language
English
Abstract
An elite cultivar of W. somnifera – WSR with desired attributes exhibiting uniform chemical profile and better adaptability in varied environment has been developed by Indian Institute of Integrative Medicine (IIIM), Jammu.
The cultivar is consistently hyper-productive in root/leaf biomass and phytochemical attributes and received commercial acceptability in India.
A highly efficient in vitro micropropagation system has been developed for this elite cultivar for mass propagation.
Field performance, LC-MS based Withanolide profile and Randomly Amplified Polymorphic DNA (RAPD) marker based analysis of micropropagated plants confirmed clonal fidelity of tissue culture raised plants.
Adoption of presently developed in vitro strategy for propagation of cultivar WSR would help to provide uniform planting material which is highly desirable from pharmacological point of view to maintain uniformity and efficacy of herbal drug preparations utilizing Indian Ginseng.
The cultivar is consistently hyper-productive in root/leaf biomass and phytochemical attributes and received commercial acceptability in India.
A highly efficient in vitro micropropagation system has been developed for this elite cultivar for mass propagation.
Field performance, LC-MS based Withanolide profile and Randomly Amplified Polymorphic DNA (RAPD) marker based analysis of micropropagated plants confirmed clonal fidelity of tissue culture raised plants.
Adoption of presently developed in vitro strategy for propagation of cultivar WSR would help to provide uniform planting material which is highly desirable from pharmacological point of view to maintain uniformity and efficacy of herbal drug preparations utilizing Indian Ginseng.
Publication
Authors
S. Mallubhotla, A. Ahuja, A. Kumar, R.D. Sharma, V. Verma, K.A. Suri, S.P. Vij
Keywords
ashwagandha, acclimatization, genetic fidelity, in vitro propagation, steroidal lactones
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