Articles
IN VITRO APPROACHES FOR CONSERVATION OF DAHLIA (DAHLIA VARIABILIS L.)
Article number
865_57
Pages
387 – 391
Language
English
Abstract
Dahlia (Dahlia variabilis L.) is one of the popular bulbous flower crops in most gardens of the world.
Dahlias are extensively used in exhibition, garden display and decoration.
The available diversity with respect to floral characters is worth conserving.
In situ field conservation is costly, labour intensive, prone to attack of pests and diseases and require more area.
However, in vitro conservation technique offers the best alternative methods for germplasm conservation.
An experiment was, therefore, conducted to standardize the growth and storability protocol for in vitro conservation of dahlia micro shoots using different concentrations of mannitol, alar (B-9) and maleic hydrazide.
Micro shoots cultured on MS medium supplemented with maleic hydrazide at 40 mg/L recorded a significantly higher number of days (27.5) for bud sprouting, least shoot length (4.25 cm), maximum storage period (134.00 days) and good percent recovery (83.00%) followed by MS medium supplemented with alar (B-9) at 40 mg/L (higher number of days for bud sprouting (22.70), least shoot length (3.40 cm), maximum storage period (129.00 days) and good percent recovery (91.00%). Next best treatment was mannitol at 20 gm/L which registered significantly higher number of days (29.20) for bud sprouting, least shoot length (4.80 cm), maximum storage period (125.00 days) and good percent recovery (89.00%).
Dahlias are extensively used in exhibition, garden display and decoration.
The available diversity with respect to floral characters is worth conserving.
In situ field conservation is costly, labour intensive, prone to attack of pests and diseases and require more area.
However, in vitro conservation technique offers the best alternative methods for germplasm conservation.
An experiment was, therefore, conducted to standardize the growth and storability protocol for in vitro conservation of dahlia micro shoots using different concentrations of mannitol, alar (B-9) and maleic hydrazide.
Micro shoots cultured on MS medium supplemented with maleic hydrazide at 40 mg/L recorded a significantly higher number of days (27.5) for bud sprouting, least shoot length (4.25 cm), maximum storage period (134.00 days) and good percent recovery (83.00%) followed by MS medium supplemented with alar (B-9) at 40 mg/L (higher number of days for bud sprouting (22.70), least shoot length (3.40 cm), maximum storage period (129.00 days) and good percent recovery (91.00%). Next best treatment was mannitol at 20 gm/L which registered significantly higher number of days (29.20) for bud sprouting, least shoot length (4.80 cm), maximum storage period (125.00 days) and good percent recovery (89.00%).
Publication
Authors
G. Shivayogeppa, J. Dinakar Adiga, G. Prabhuling, B.S. Reddy, B.N. Sathyanarayana
Keywords
in vitro, in situ, micro-shoots, mannitol, alar, maleic hydrazid
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