Articles
MICROPROPAGATION STUDIES ON IMPORTANT FOREST TREES OF HIGH IMPORTANCE: AN EFFORT FOR LARGE-SCALE COMMERCIAL MULTIPLICATION AND THEIR FIELD PLANTATION
Article number
865_32
Pages
255 – 264
Language
English
Abstract
The present project is taken up with the aim to develop micropropagation protocols for large and commercial scale multiplication of highly important forest trees, i.e., Eucalyptus hybrids.
Two Eucalyptus hybrids namely as FRI-5 (Eucalyptus camaldulensis Dehn × Eucalytpus tereticornis Sm.) and FRI-14 (Eucalyptus torelliana F.V. Muell × Eucalyptus citriodora Hook) were selected for the present course of investigation with the aim to develop mass scale micropropagation technique using nodal segments as explants from the mature trees (3032 years old) of both the hybrids.
These Eucalyptus hybrids were produced by Forest Research Institute, Dehradun in the early 1960-70s though controlled crossing are available in very limited number and need multiplication true to type for field-testing to ascertain its superiority under varied agro-climatic conditions.
These hybrids showed 35 superior in terms of total biomass and wood volume when compared to their parents.
Cultures were established and multiplied on MS medium supplemented different concentration of BAP either alone or in with combination of auxins i.e.
IBA/NAA. MS medium was found to be effective and suitable for all the experiments during plant production.
Eighty-five to 92% rooting was achieved on half strength MS medium supplemented with IBA. Ninety-five to 98% plantlets survival was obtained after hardening and acclimatization prior to field transfer.
Parameters like height, DBH, clear bole length and self pruning capability of both the hybrid were recorded up to three years to identify the suitability of these hybrids in different varied climatic zones of Uttarakhand.
Two Eucalyptus hybrids namely as FRI-5 (Eucalyptus camaldulensis Dehn × Eucalytpus tereticornis Sm.) and FRI-14 (Eucalyptus torelliana F.V. Muell × Eucalyptus citriodora Hook) were selected for the present course of investigation with the aim to develop mass scale micropropagation technique using nodal segments as explants from the mature trees (3032 years old) of both the hybrids.
These Eucalyptus hybrids were produced by Forest Research Institute, Dehradun in the early 1960-70s though controlled crossing are available in very limited number and need multiplication true to type for field-testing to ascertain its superiority under varied agro-climatic conditions.
These hybrids showed 35 superior in terms of total biomass and wood volume when compared to their parents.
Cultures were established and multiplied on MS medium supplemented different concentration of BAP either alone or in with combination of auxins i.e.
IBA/NAA. MS medium was found to be effective and suitable for all the experiments during plant production.
Eighty-five to 92% rooting was achieved on half strength MS medium supplemented with IBA. Ninety-five to 98% plantlets survival was obtained after hardening and acclimatization prior to field transfer.
Parameters like height, DBH, clear bole length and self pruning capability of both the hybrid were recorded up to three years to identify the suitability of these hybrids in different varied climatic zones of Uttarakhand.
Publication
Authors
S. Sharma, N. Rathi, S. Nautiyal, I.D. Arya
Keywords
Eucalyptus hybrids FRI-5 & FRI-14, MS, field plantation, DBH
Online Articles (60)