Articles
THE EFFECT OF BENZYL ADENINE (BA) HORMONE AND EXPLANTS TYPE ON ESTABLISHMENT AND PROLIFERATION OF IRANIAN DWARFING APPLE ROOTSTOCK ‘AZAYESH’ UNDER IN VITRO CONDITION
Article number
865_17
Pages
135 – 140
Language
English
Abstract
Plant tissue culture techniques have efficient utility in micro propagation, disease free stock and helpful to genetic improvement programs.
In this study, the effects of Benzyl Adenine (BA) and explants type on establishment and proliferation of apples dwarfing rootstock (Azayesh) were investigated.
Two concentration levels of BA (4, 2 mg/L), two types of explants (sucker, main shoot) and four sampling seasons were compared with each other.
MS medium under light (16 h) and temperature (22±2°C) condition was selected.
In all experiments, the best responses belong to those explants which derived from spring and cultured on MS with 4 mg/L (BA). Collected explants at summer, fall and winter, showed high percentage of necrosis.
Explants derived from sucker, possessing more proliferation rate than explants obtained from shoots.
In this study, the effects of Benzyl Adenine (BA) and explants type on establishment and proliferation of apples dwarfing rootstock (Azayesh) were investigated.
Two concentration levels of BA (4, 2 mg/L), two types of explants (sucker, main shoot) and four sampling seasons were compared with each other.
MS medium under light (16 h) and temperature (22±2°C) condition was selected.
In all experiments, the best responses belong to those explants which derived from spring and cultured on MS with 4 mg/L (BA). Collected explants at summer, fall and winter, showed high percentage of necrosis.
Explants derived from sucker, possessing more proliferation rate than explants obtained from shoots.
Publication
Authors
S. Kianamiri, M. Hassani
Keywords
apple, proliferation, cytokinine, MS medium, necrosis
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