Articles
ACCUMULATION OF HEAVY METALS BY SELECTED WILLOW CLONES
Article number
865_40
Pages
297 – 300
Language
English
Abstract
Micropropagated plants derived from 4 selected clones of the willows (S. alba × S. blanda, S. miyabeana, S. viminalis and S. elbursensis) in the field trials were used to determine the uptake of the Cd and Pb.
The plantlets were cultured in sterile conditions in hydroponic systems: 10 times diluted MS medium (Murashige and Skoog, 1962) supplied by 0.5 mM of Cd(CH3COO)2.3H2O and the same concentrations of Pb(CH3COO)2.3H2O, pH adjusted to 5.6 by 1.0 N KOH, and in 10 times diluted MS solution (controls). Concentrations of heavy metals in stem parts and roots were determined using the ICP-OES method.
The differences in accumulation capacity of Cd and Pb were identified in willow species.
It could be concluded that selected willow clones displayed a high efficacy of accumulation of Cd and Pb in hydroponics conditions and could represent a prospective tree for phytoremediation of polluted soils.
The plantlets were cultured in sterile conditions in hydroponic systems: 10 times diluted MS medium (Murashige and Skoog, 1962) supplied by 0.5 mM of Cd(CH3COO)2.3H2O and the same concentrations of Pb(CH3COO)2.3H2O, pH adjusted to 5.6 by 1.0 N KOH, and in 10 times diluted MS solution (controls). Concentrations of heavy metals in stem parts and roots were determined using the ICP-OES method.
The differences in accumulation capacity of Cd and Pb were identified in willow species.
It could be concluded that selected willow clones displayed a high efficacy of accumulation of Cd and Pb in hydroponics conditions and could represent a prospective tree for phytoremediation of polluted soils.
Publication
Authors
J. Malá, H. Cvrčková, P. Máchová
Keywords
metal uptake, hydroponics, micropropagated plantlets
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