Articles
MOLECULAR ANALYSIS OF MICROPROPAGATED APPLE ROOTSTOCK MM111 USING ISSR MARKERS FOR ASCERTAINING CLONAL FIDELITY
Article number
865_8
Pages
73 – 80
Language
English
Abstract
Inter simple sequence markers (ISSR) were used to assess the genetic stability of micropropagated plants regenerated through axillary buds of clonal apple (Malus × domestica Borkh.) rootstock MM111 after twenty second passages.
A total of 24 primers were initially screened and finally 15 were chosen for the present study to analyse genomic DNA from mother plants and in vitro plant material.
A total of 147 amplification products were generated with an average of 10 bands per primer.
The outlier M7 was also taken along with TC raised progenies of MM111 to rule out the possibility that the invariant banding pattern was on account of inefficiency of ISSR primers in detecting variations.
A homogenous amplification profile was observed for all the micropropagated plants.
The results confirmed the clonal fidelity of the tissue culture-raised MM111 plantlets and corroborated the fact that axillary multiplication is the safest mode for multiplication of true-to-type plants.
A total of 24 primers were initially screened and finally 15 were chosen for the present study to analyse genomic DNA from mother plants and in vitro plant material.
A total of 147 amplification products were generated with an average of 10 bands per primer.
The outlier M7 was also taken along with TC raised progenies of MM111 to rule out the possibility that the invariant banding pattern was on account of inefficiency of ISSR primers in detecting variations.
A homogenous amplification profile was observed for all the micropropagated plants.
The results confirmed the clonal fidelity of the tissue culture-raised MM111 plantlets and corroborated the fact that axillary multiplication is the safest mode for multiplication of true-to-type plants.
Publication
Authors
H. Pathak, V. Dhawan
Keywords
Malus × domestica, RAPD, somaclonal variations, micropropagation, axillary multiplication, meristem, molecular markers
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