Articles
IN VITRO REGENERATION OF PINUS SPP. ADULT TREES: NEW METHOD FOR OBTAINING CLONAL PLANTS
Article number
865_52
Pages
361 – 365
Language
English
Abstract
In this study, carried out from November 2006 to March 2007, shoot buds were collected from twenty Pinus pinaster Ait. and Pinus sylvestris L. adult trees (>15 years old). Buds were transversely cut into 0.51 cm slices and cultured (4 explants/Petri dish) on different media [DCR, WP and modified LP] supplemented with cytokinins [6-benzyladenine (BA), meta-topolin (mT), zeatin (Z), and thidiazuron (Tdz)], at two concentrations.
The highest organogenic response of P. pinaster; i.e., axillary shoots formation ability, was obtained on DCR medium supplemented with 25 mmol/L Z and mT, and 25 or 50 mmol/L BA. All the shoots that were cultured on DCR with 25 mmol/L BA developed as healthy and well rooted plantlets.
On the other hand, the highest organogenic response of buds from P. sylvestris adult trees was shown equality on DCR and WP medium supplemented with 25 mmol/L meta-topolin.
The ability to regenerate adult trees represents a significant progress in the application of biotechnology to forest trees improvement programs and it opens the possibility of using micropropagated selected trees in agro-forestry areas under biotic or abiotic stress.
The highest organogenic response of P. pinaster; i.e., axillary shoots formation ability, was obtained on DCR medium supplemented with 25 mmol/L Z and mT, and 25 or 50 mmol/L BA. All the shoots that were cultured on DCR with 25 mmol/L BA developed as healthy and well rooted plantlets.
On the other hand, the highest organogenic response of buds from P. sylvestris adult trees was shown equality on DCR and WP medium supplemented with 25 mmol/L meta-topolin.
The ability to regenerate adult trees represents a significant progress in the application of biotechnology to forest trees improvement programs and it opens the possibility of using micropropagated selected trees in agro-forestry areas under biotic or abiotic stress.
Publication
Authors
N. De Diego, I.A. Montalbán, P. Moncaleán
Keywords
Pinus pinaster, Pinus sylvestris, in vitro, bud, shoot, explant, root, cytokinin
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