Articles
EASY CLONING AND ACCLIMATIZATION OF REBUTIA MUSCULA
Article number
865_34
Pages
269 – 271
Language
English
Abstract
The objective of this investigation was to establish an effective and simple methodology for the germination and propagation in vitro of this species.
Seeds of mature fruits from a nursery were used.
They were sown aseptically on a free hormone MS medium.
The shoots obtained were transplanted to 3 multiplication treatments: half-strength MS free hormone medium (T0), half-strength MS medium added with 4.44 µM BA + 0.053 µM GA3 (T1), and complete MS medium plus 2.22 µM BA + 2.69 µM NAA + 1.87 µM GA3. Two explant orientations were probed, some shoots stood upright on the medium while others were placed horizontally on the medium.
The experiment was a randomized block design with 10 replications.
The number of buds was evaluated after the 6 weeks of culture.
For the vertical position of the explants there were no significant differences between the treatments of multiplication 1 and 2 obtaining an average of 25 and 22.6, respectively.
For the horizontal position, treatment 2 was better, with a mean of 19.1 shoots by explants.
The vertical position produced a greater mean number of buds 23.8. Shoots has a low in vitro rooting rate, but have a good surviving during the acclimatization stage.
Seeds of mature fruits from a nursery were used.
They were sown aseptically on a free hormone MS medium.
The shoots obtained were transplanted to 3 multiplication treatments: half-strength MS free hormone medium (T0), half-strength MS medium added with 4.44 µM BA + 0.053 µM GA3 (T1), and complete MS medium plus 2.22 µM BA + 2.69 µM NAA + 1.87 µM GA3. Two explant orientations were probed, some shoots stood upright on the medium while others were placed horizontally on the medium.
The experiment was a randomized block design with 10 replications.
The number of buds was evaluated after the 6 weeks of culture.
For the vertical position of the explants there were no significant differences between the treatments of multiplication 1 and 2 obtaining an average of 25 and 22.6, respectively.
For the horizontal position, treatment 2 was better, with a mean of 19.1 shoots by explants.
The vertical position produced a greater mean number of buds 23.8. Shoots has a low in vitro rooting rate, but have a good surviving during the acclimatization stage.
Publication
Authors
D. Orea Coria, A. Medrano Valverde
Keywords
Cactaceae, micropropagation, explant position
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