Articles
BERRY BIOREACTOR MICROPROPATION AND MOLECULAR ANALYSIS FOR GENETIC FIDELITY
Article number
865_4
Pages
43 – 51
Language
English
Abstract
An improved understanding of the important role of dietary fruit in maintaining human health has led to a dramatic increase in global berry demand and production in recent years.
While berry fruit crops have long enjoyed huge popularity among consumers, tremendous progress in plant tissue culture and berry crop micropropagation has occurred.
Of particular significance has been the evolution of the technology permitting multiplication of berry plants through bioreactor micropropagation.
Automation of micropropagation in bioreactors has been advanced as a possible way of reducing propagation cost.
However, optimal plant production depends upon better understanding of physiological and biochemical responses of plant to the signals of liquid culture microenvironment.
Clonal fidelity can be a serious problem and strategies have been developed in order to reduce the variation to manageable levels.
Molecular markers such as RAPDs, RFLPs, AFLPs, DAFs, SCARs, SSRs and ISSRs have been introduced in tissue culture research and can potentially be used in various facets of pertinent studies with berry crops.
This review focuses on the bioreactor systems used for in vitro culture of berry crops and employment of molecular markers in micropropagated berry plants for the assessment of genetic fidelity, uniformity, stability, and true-to-typeness among donor plants and tissue culture regenerants.
The relevant literature is reviewed and the relative merits and shortcomings of the various molecular markers applied are presented with an emphasis on the nature of tissue culture-induced variation.
While berry fruit crops have long enjoyed huge popularity among consumers, tremendous progress in plant tissue culture and berry crop micropropagation has occurred.
Of particular significance has been the evolution of the technology permitting multiplication of berry plants through bioreactor micropropagation.
Automation of micropropagation in bioreactors has been advanced as a possible way of reducing propagation cost.
However, optimal plant production depends upon better understanding of physiological and biochemical responses of plant to the signals of liquid culture microenvironment.
Clonal fidelity can be a serious problem and strategies have been developed in order to reduce the variation to manageable levels.
Molecular markers such as RAPDs, RFLPs, AFLPs, DAFs, SCARs, SSRs and ISSRs have been introduced in tissue culture research and can potentially be used in various facets of pertinent studies with berry crops.
This review focuses on the bioreactor systems used for in vitro culture of berry crops and employment of molecular markers in micropropagated berry plants for the assessment of genetic fidelity, uniformity, stability, and true-to-typeness among donor plants and tissue culture regenerants.
The relevant literature is reviewed and the relative merits and shortcomings of the various molecular markers applied are presented with an emphasis on the nature of tissue culture-induced variation.
Publication
Authors
S.C. Debnath
Keywords
in vitro culture, liquid medium, molecular marker, Rubus, strawberry, Vaccinium
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