Articles
EFFECTS OF HORMONES, EXPLANT AND MEDIA ON ROOTING AND STABILIZATION OF BERMUDAGRASS (CYNODON DACTYLON) IN TISSUE CULTURE
Article number
865_18
Pages
141 – 148
Language
English
Abstract
Nowadays, tissue culture technique is not only used for micropropagation but it is also the bases of biotechnology and breeding programs.
Rooting and stabilization of explants is an important stage after proliferation which accomplished in vitro or in vivo conditions.
Explants show considerable changes during transferring to the outdoors if sufficient caution is not received.
Turfgrass is planted as major vegetative groundcover in many landscapes, especially in urban regions.
Among turfgrasses, bermudagrass (Cynodon dactylon) is as a main portion in sport mixture turfgrass.
The aims of this research were to find the best concentration of naphthalene acetic acid (NAA) and also to determine an appropriate media for adaptation stage of bermudagrass.
We used different concentration of NAA (5, 10, 15 and 20 mg L-1) to induced root and different of composition of pumice, vermiculite and loam for stabilization stage.
According to the results, the highest root production was obtained by using 5 mg L-1 NAA in in vitro conditions.
High concentration of auxin inhibited morphogenesis during tissue culture.
NAA usually used for inducing root.
Composition of loam plus vermiculite and pumice caused to highest adaptation of explants in in vivo conditions.
Also, equal composition of horticultural soil, vermiculite and pumice do an appropriate media for root growth.
Rooting and stabilization of explants is an important stage after proliferation which accomplished in vitro or in vivo conditions.
Explants show considerable changes during transferring to the outdoors if sufficient caution is not received.
Turfgrass is planted as major vegetative groundcover in many landscapes, especially in urban regions.
Among turfgrasses, bermudagrass (Cynodon dactylon) is as a main portion in sport mixture turfgrass.
The aims of this research were to find the best concentration of naphthalene acetic acid (NAA) and also to determine an appropriate media for adaptation stage of bermudagrass.
We used different concentration of NAA (5, 10, 15 and 20 mg L-1) to induced root and different of composition of pumice, vermiculite and loam for stabilization stage.
According to the results, the highest root production was obtained by using 5 mg L-1 NAA in in vitro conditions.
High concentration of auxin inhibited morphogenesis during tissue culture.
NAA usually used for inducing root.
Composition of loam plus vermiculite and pumice caused to highest adaptation of explants in in vivo conditions.
Also, equal composition of horticultural soil, vermiculite and pumice do an appropriate media for root growth.
Publication
Authors
M. Taghizadeh, M. Kafi, R. Fatahi
Keywords
bermudagrass, NAA, tissue culture media, acclimatization, rooting
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