Articles
ANATOMY OF ROOT DEVELOPMENT IN TISSUE CULTURE RAISED TERMINALIA BELLERICA ROXB. AND ITS SIGNIFICANCE IN HARDENING
Article number
865_15
Pages
123 – 127
Language
English
Abstract
Anatomy of in vitro adventitious root development in Terminalia bellerica Roxb. microshoots grown on MS medium containing ¼th strength of MS salts and supplemented with 0.1 mg L-1 IBA was studied.
Transverse section of shoot on day one showed normal shoot anatomy.
By day 3, initiation of root primordia was observed.
After day 6, root primordia underwent further differentiation, and development of root-to-shoot xylem connection was observed.
Further development and emergence of adventitious roots was observed on day 8 to 10. The anatomical studies revealed that in vitro developed root system had proper vascular connection with shoot which was significant for hardening, plantlet growth and higher ex vitro survival.
Transverse section of shoot on day one showed normal shoot anatomy.
By day 3, initiation of root primordia was observed.
After day 6, root primordia underwent further differentiation, and development of root-to-shoot xylem connection was observed.
Further development and emergence of adventitious roots was observed on day 8 to 10. The anatomical studies revealed that in vitro developed root system had proper vascular connection with shoot which was significant for hardening, plantlet growth and higher ex vitro survival.
Publication
Authors
R.K. Suthar, S.D. Purohit
Keywords
micropropagation, in vitro rooting, developmental anatomy, ex vitro survival medicinal plant
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