Articles
IN VITRO ROOTING AND HARDENING IN APPLE ROOTSTOCK EMLA111 – INFLUENCE OF SOME FACTORS
Article number
865_47
Pages
339 – 344
Language
English
Abstract
An efficient and reliable procedure for rooting and hardening of in vitro raised shoots of EMLA111 has been developed.
Influence of different concentrations of IBA/NAA, solid/liquid medium, role of dark/light treatment and phloroglucinol (PG) was seen on rooting.
Nearly 75% rooting was obtained on ½ MS with 0.10.5 mg/L IBA, 2% sucrose and 0.4% agar in one step method.
However, successful rooting was related to the exposure of shoots to IBA for few days followed by transfer to auxin free media (two step method). A synergistic effect of IBA and phloroglucinol in light on rooting was observed.
Rooted plantlets were successfully transferred to coco-peat for acclimatization.
The hardened plants were transferred to open field conditions at different locations.
Ninety-five to 100 percent success was found in MarchApril and SeptemberNovember.
Influence of different concentrations of IBA/NAA, solid/liquid medium, role of dark/light treatment and phloroglucinol (PG) was seen on rooting.
Nearly 75% rooting was obtained on ½ MS with 0.10.5 mg/L IBA, 2% sucrose and 0.4% agar in one step method.
However, successful rooting was related to the exposure of shoots to IBA for few days followed by transfer to auxin free media (two step method). A synergistic effect of IBA and phloroglucinol in light on rooting was observed.
Rooted plantlets were successfully transferred to coco-peat for acclimatization.
The hardened plants were transferred to open field conditions at different locations.
Ninety-five to 100 percent success was found in MarchApril and SeptemberNovember.
Publication
Authors
M. Modgil, R. Gupta, M. Thakur
Keywords
in vitro rooting, hardening of apple rootstocks, micropropagation, photoperiod
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