Articles
EFFECT OF INORGANIC FERTILIZER, MYCORRHIZA AND FARM YARD MANURE ON GROWTH OF MICROPROPAGATED APPLE ROOTSTOCK M.7, FOR THE PRODUCTION OF GRAFTABLE PLANTS IN ONE YEAR
Article number
865_26
Pages
209 – 215
Language
English
Abstract
Micropropagation in comparison with the vegetative propagation methods, helps to increase the production in shorter periods of time.
But, in case of micropropagated apple rootstocks it still takes 2 years for the plants to attain a thickness appropriate for grafting.
In this context it was aimed to evaluate the effect of inorganic fertilizer, mycorrhiza (AM, Glomus intraradices) and farmyard manure (FYM) on growth of micropropagated apple rootstock M.7. Plants acclimatized for one month were transplanted into pots and were maintained in nursery for 6 months.
Eight treatments tested include: soil (control); soil + AM; soil + FYM (4:1v/v); soil + N-P-K; soil + N-P-K + AM; soil + FYM + AM; soil + FYM+ N-P-K and soil + FYM + NPK+AM. N-P-K fertigation was done 8 times at an interval of 14 days using 0.2% 19-19-19 N-P-K fertilizer.
The variables analyzed involve survival percentage, shoot height, number of leaves, number of new leaves, percentage root colonization, root length, plant biomass.
Root colonization was observed in all the treatments and was not effected by addition of FYM and NPK. Nevertheless, AM fungi had no significant effect on growth in any of the treatments.
Soil + FYM + NPK and soil + FYM + NPK + AM out of eight treatments, enhanced shoot height (4 fold), number of leaves (2 fold) and plant biomass significantly as compared to the control.
None of the treatments had a significant effect on survival percentage, root length and emergence of new leaves.
Considering that 100% of plants treated with these two treatments attained graftable thickness in 6 months as against 8% plants in the control, it can be successfully applied for the production of grafted apple trees in lesser time.
But, in case of micropropagated apple rootstocks it still takes 2 years for the plants to attain a thickness appropriate for grafting.
In this context it was aimed to evaluate the effect of inorganic fertilizer, mycorrhiza (AM, Glomus intraradices) and farmyard manure (FYM) on growth of micropropagated apple rootstock M.7. Plants acclimatized for one month were transplanted into pots and were maintained in nursery for 6 months.
Eight treatments tested include: soil (control); soil + AM; soil + FYM (4:1v/v); soil + N-P-K; soil + N-P-K + AM; soil + FYM + AM; soil + FYM+ N-P-K and soil + FYM + NPK+AM. N-P-K fertigation was done 8 times at an interval of 14 days using 0.2% 19-19-19 N-P-K fertilizer.
The variables analyzed involve survival percentage, shoot height, number of leaves, number of new leaves, percentage root colonization, root length, plant biomass.
Root colonization was observed in all the treatments and was not effected by addition of FYM and NPK. Nevertheless, AM fungi had no significant effect on growth in any of the treatments.
Soil + FYM + NPK and soil + FYM + NPK + AM out of eight treatments, enhanced shoot height (4 fold), number of leaves (2 fold) and plant biomass significantly as compared to the control.
None of the treatments had a significant effect on survival percentage, root length and emergence of new leaves.
Considering that 100% of plants treated with these two treatments attained graftable thickness in 6 months as against 8% plants in the control, it can be successfully applied for the production of grafted apple trees in lesser time.
Publication
Authors
H. Pathak, V. Dhawan
Keywords
micropropagation, colonization, grafting, AMF, acclimatized, fertigation
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