Articles
FIRST REPORT ON CALLUS INDUCTION IN ASHOKA (SARACA INDICA): AN IMPORTANT MEDICINAL PLANT
Article number
865_56
Pages
383 – 386
Language
English
Abstract
Leaves cultured on Murashige and Skoog (MS) media supplemented with BAP (2 mg/L) and NAA (0.5 mg/L) showed calli formation in 29.4% explants.
When cultured on half strength MS media (2 mg/L BAP and 0.5 mg/L NAA), 13.3% explants could produce calli.
Hypocotyls segments cultured on full strength MS media showed good callusing response (52.6%). Culture of explants on the half strength MS media produced calli in 77.8% of explants.
When segments of epicotyls were placed on full strength MS media, a callusing response of 83.3% was observed.
Segments when were placed on half strength MS media, a maximum response of 88.9% was observed.
The calli failed to differentiate into shoots when sub-cultured on shoot induction media.
When cultured on half strength MS media (2 mg/L BAP and 0.5 mg/L NAA), 13.3% explants could produce calli.
Hypocotyls segments cultured on full strength MS media showed good callusing response (52.6%). Culture of explants on the half strength MS media produced calli in 77.8% of explants.
When segments of epicotyls were placed on full strength MS media, a callusing response of 83.3% was observed.
Segments when were placed on half strength MS media, a maximum response of 88.9% was observed.
The calli failed to differentiate into shoots when sub-cultured on shoot induction media.
Publication
Authors
A.A. Waman, K. Umesha, B.N. Sathyanarayana
Keywords
leaves, hypocotyls, epicotyls, powdery callus, in vitro
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