Articles
IN VITRO CULTURE OF PERESKIA BLEO
Article number
829_13
Pages
99 – 104
Language
English
Abstract
Pereskia bleo KUNTH is known locally in Malaysia as pokok jarum tujuh bilah. It is a leafy cactus which has many medicinal properties.
The leaf of this plant has been used in Malaysian traditional medicine for the prevention and treatment of breast cancer.
Callus, cell suspension, and hairy root cultures of Pereskia bleo have been successfully induced.
Callus culture was induced in MS (Murashige and Skoog) medium supplemented with various concentrations of 2,4-D. MS medium supplemented with 2.26 µM 2,4-D appeared to be the most effective to induce callus formation.
Friable, soft, and rapidly-growing callus was obtained from stem explants on an MS medium supplemented with 2.26 µM 2,4-D. Following that, cell suspension cultures could be induced from this callus and used to obtain fast-growing cells.
By comparison, MS medium supplemented with lower concentrations of 2,4-D resulted in the production of light yellow cell suspensions that grew rapidly and produced a high biomass.
Higher concentrations of 2,4-D resulted in the production of dark brown cells with slow growth in cell suspension.
Cell biochemical activity and cell growth rate of Pereskia bleo cell suspension were differentially affected by various concentrations of 2,4-D. Putative hairy root cultures were produced in Pereskia bleo by using a number of Agrobacterium rhizogenes strains (ATCC 15834, LBA 402, TR 105, and 8196). The callus, cell suspension, and putative hairy root cultures obtained from this experiment can be used to analyze the phytochemical and biological activities within these cultures with a view to produce the anticancer chemicals.
The leaf of this plant has been used in Malaysian traditional medicine for the prevention and treatment of breast cancer.
Callus, cell suspension, and hairy root cultures of Pereskia bleo have been successfully induced.
Callus culture was induced in MS (Murashige and Skoog) medium supplemented with various concentrations of 2,4-D. MS medium supplemented with 2.26 µM 2,4-D appeared to be the most effective to induce callus formation.
Friable, soft, and rapidly-growing callus was obtained from stem explants on an MS medium supplemented with 2.26 µM 2,4-D. Following that, cell suspension cultures could be induced from this callus and used to obtain fast-growing cells.
By comparison, MS medium supplemented with lower concentrations of 2,4-D resulted in the production of light yellow cell suspensions that grew rapidly and produced a high biomass.
Higher concentrations of 2,4-D resulted in the production of dark brown cells with slow growth in cell suspension.
Cell biochemical activity and cell growth rate of Pereskia bleo cell suspension were differentially affected by various concentrations of 2,4-D. Putative hairy root cultures were produced in Pereskia bleo by using a number of Agrobacterium rhizogenes strains (ATCC 15834, LBA 402, TR 105, and 8196). The callus, cell suspension, and putative hairy root cultures obtained from this experiment can be used to analyze the phytochemical and biological activities within these cultures with a view to produce the anticancer chemicals.
Authors
N. Jaafar Sidik, M.S. Norihan, K. Shafii
Keywords
callus, cell suspension, putative hairy root, Pereskia bleo
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