Articles
CHARACTERIZATION OF CHLOROSIS-RESISTANT APPLE MUTANTS OF TRANSGENIC FRO2
Article number
829_36
Pages
251 – 257
Language
English
Abstract
In order to increase ferric chelate in apple plants and increase the chlorosis-resistant capacity of plants growing in saline-alkaline soil, transgenic apple plants expressing ferric chelate reductase gene FRO2 were investigated.
Under iron deficiency conditions, the FRO2 reductase activity in the root tissues of the transformed plants increased 2.2 times compared to the control plants without iron deficiency.
The ferric chelate content in the leaves of the transformed plants was 2.1 times higher than the control plants.
The leaves of the transformed plants remained green and observed chlorosis was lower than in the control plants.
In Fe(II)-sufficient soil, the gene expression of the FRO2 plants increased 1.88 times and the content of ferric chelate was 1.7 times higher than those of control plants in roots and leaves, respectively.
The ferric chelate reductase activity of roots and leaves of the control apple plants was lower and could not be induced effectively under iron deficiency conditions, but the reductase activity in roots of the FRO2 plants was induced to higher levels.
These results suggest that the FRO2 transformed apple plants can be cultivated in saline-alkaline soils and in iron deficient soils in order to overcome chlorosis.
Under iron deficiency conditions, the FRO2 reductase activity in the root tissues of the transformed plants increased 2.2 times compared to the control plants without iron deficiency.
The ferric chelate content in the leaves of the transformed plants was 2.1 times higher than the control plants.
The leaves of the transformed plants remained green and observed chlorosis was lower than in the control plants.
In Fe(II)-sufficient soil, the gene expression of the FRO2 plants increased 1.88 times and the content of ferric chelate was 1.7 times higher than those of control plants in roots and leaves, respectively.
The ferric chelate reductase activity of roots and leaves of the control apple plants was lower and could not be induced effectively under iron deficiency conditions, but the reductase activity in roots of the FRO2 plants was induced to higher levels.
These results suggest that the FRO2 transformed apple plants can be cultivated in saline-alkaline soils and in iron deficient soils in order to overcome chlorosis.
Authors
Jinghui Yang, Yanjun Liu, Yudong Liu, Weiyu Zhang, Junxuan Huang, Jianke Li, Chang Cheng Du, Zhuo Sui, Gefu Wang-Pruski
Keywords
transgenic apple, iron deficiency, ferric chelate reductase, chlorosis symptom
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