Home > VI International Symposium on In Vitro Culture and Horticultural Breeding > EFFECTS OF VARIOUS SUCROSE CONCENTRATION AND ANCYMIDOL ON SHOOT ELONGATION AND WATER STATUS OF ASPARAGUS IN VITRO

Articles

EFFECTS OF VARIOUS SUCROSE CONCENTRATION AND ANCYMIDOL ON SHOOT ELONGATION AND WATER STATUS OF ASPARAGUS IN VITRO

Article number

829_45

Pages

299 – 303

Language

English

Abstract

We investigated the effect of osmotic stress on elongation and the water status of tissue-cultured asparagus (Asparagus officinalis L. cv. UC157F1). The shoot tips of asparagus were excised and transplanted to MS media containing 15-60 g L^-1 sucrose concentration.
There was no apparent root development; water potential gradients between elongation region (shoot tip) and mature region (base of stem) were correlated to shoot elongation rate.
When the media contained 15 g L^-1 of sucrose, the gradient and elongation rate was the highest.
This result suggested that measuring water potential gradient may be used as a useful indicator of ascertaining optimum medium condition for tissue-cultured plants.
Additionally, we investigated the effect of ancymidol, which improved differentiation and development of asparagus root on shoot elongation and water status.

Publication

VI International Symposium on In Vitro Culture and Horticultural Breeding

Authors

R. Honjo, T. Ikeda, A. Taji, A. Uragami

Keywords

growth-induced water potential, isopiestic psychrometer

Full text

https://doi.org/10.17660/ActaHortic.2009.829.45

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