DEVELOPMENT OF MICROCOLONIES IN PROTOPLAST CULTURE OF SPATHIPHYLLUM WALLISII

Somatic fusion within Araceae may be an alternative for interspecific sexual hybridisation.
In this research, Spathiphyllum ‘Daniël’ protoplasts were embedded in 100 µl agarose droplets and cultured in 5 ml liquid medium based on Kao and Michayluk (1975). The effect of several parameters was evaluated.
The initial protoplast concentration (tested between 10⁴ and 10⁵/ml) was positively correlated to the total amount of initial divisions.
A beneficial effect on microcolony formation was seen after adding glucose to the regeneration medium at high protoplast density.
Microcolonies consisting out of ca. 30-50 cells were observed, which was not been reported previously for Spathiphyllum. However, plants could not yet be regenerated.
These results are however an indication of the importance of carbohydrate concentration combined with high protoplast density for the regeneration of Spathiphyllum protoplasts.

VI International Symposium on In Vitro Culture and Horticultural Breeding

T. Eeckhaut, B. Duquenne, P. Laksmanan, J. Van Huylenbroeck

interspecific hybrids, polyploids, Rhododendron, sport regeneration, unreduced gametes

https://doi.org/10.17660/ActaHortic.2009.829.5