Articles
AGROBACTERIUM TUMEFACIENS-MEDIATED TRANSFORMATION OF ONCIDIUM AND ODONTOGLOSSUM WITH THE MUTATED ETHYLENE RECEPTOR ETR1-1
Article number
829_21
Pages
149 – 154
Language
English
Abstract
Ornamental orchids Oncidium and Odontoglossum are sensitivite to ethylene, and their senescence process is initiated very fast.
Ethylene receptor mutant gene etr1-1 from Arabidopsis is known to prevent ethylene induced senescence, which can occur during transport or/and storage of plants.
Therefore, the objective of our project was to transform Oncidium and Odontoglossum with etr1-1 under control of a flower-specific promoter to achieve a prolonged shelf life.
Establishment of an efficient protocol for in vitro regeneration via somatic embryogenesis and Agrobacterium-mediated transformation was necessary for achievement of our aim.
Starting from in vitro shoot cultures, we established a reliable protocol for the regeneration of protocorm-like-bodies (PLBs). By applying this regeneration protocol we tested different parameters for Agrobacterium-mediated gene transfer using PLBs in liquid culture.
Using histochemical GUS assays transformation events were assessed after two, four, and six months.
The molecular analyses of the putative transgenic plants are in progress.
Ethylene receptor mutant gene etr1-1 from Arabidopsis is known to prevent ethylene induced senescence, which can occur during transport or/and storage of plants.
Therefore, the objective of our project was to transform Oncidium and Odontoglossum with etr1-1 under control of a flower-specific promoter to achieve a prolonged shelf life.
Establishment of an efficient protocol for in vitro regeneration via somatic embryogenesis and Agrobacterium-mediated transformation was necessary for achievement of our aim.
Starting from in vitro shoot cultures, we established a reliable protocol for the regeneration of protocorm-like-bodies (PLBs). By applying this regeneration protocol we tested different parameters for Agrobacterium-mediated gene transfer using PLBs in liquid culture.
Using histochemical GUS assays transformation events were assessed after two, four, and six months.
The molecular analyses of the putative transgenic plants are in progress.
Authors
B. Raffeiner, T. Winkelmann, M. Serek
Keywords
ethylene, in vitro culture, orchids, PLBs, senescence, somatic embryogenesis
Online Articles (69)