Articles
THE EFFECT OF VARIOUS HORMONE TYPES ON IN VITRO PROPAGATION OF STRAWBERRY
Article number
829_46
Pages
305 – 308
Language
English
Abstract
The objective of this study was to assess the effect of Thidiazuron (TDZ) alone or in combination with IAA on both the in vitro shoot multiplication-index and plantlet quality of the strawberry cultivar Camarosa. We applied the following concentrations of TDZ alone (0, 0.5, 1.0, 1.5, 2.0 mg/L) or in combination with
1.0 mg/L IAA. In addition, we applied IAA at the following concentrations: 0.4, 0.8, 1.0, 1.2 mg/L together with 1.0 mg/L TDZ. In the rooting stage, different concentrations of activated charcoal (0, 2.5, 5.0 g/L) and IBA (0, 0.1, 0.4, 0.8,
1.0 mg/L) were tested.
The results demonstrated that shoot proliferation was significantly affected by TDZ alone and in combination with IAA. Our recommendation for the optimal multiplication of Camarosa is: MS medium supplemented with 1.5 mg/L TDZ and 1 mg/L IAA. Using this medium we obtained 28.46 shoots per explant.
As a result of this study, it was determined that the increased concentrations of IBA decreased the root development.
The highest root development ratio was found in media that contains activated charcoal.
1.0 mg/L IAA. In addition, we applied IAA at the following concentrations: 0.4, 0.8, 1.0, 1.2 mg/L together with 1.0 mg/L TDZ. In the rooting stage, different concentrations of activated charcoal (0, 2.5, 5.0 g/L) and IBA (0, 0.1, 0.4, 0.8,
1.0 mg/L) were tested.
The results demonstrated that shoot proliferation was significantly affected by TDZ alone and in combination with IAA. Our recommendation for the optimal multiplication of Camarosa is: MS medium supplemented with 1.5 mg/L TDZ and 1 mg/L IAA. Using this medium we obtained 28.46 shoots per explant.
As a result of this study, it was determined that the increased concentrations of IBA decreased the root development.
The highest root development ratio was found in media that contains activated charcoal.
Authors
N. Adak, L. Kaynak, M. Pekmezci, H. Gubbuk
Keywords
tissue culture, thidiazuron, IAA
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