Articles
MICROBIAL CONTAMINATION IN EXPLANTS OF BANANA CULTIVARS ‘GALIL 18’ AND ‘TROPICAL’
Article number
829_53
Pages
341 – 344
Language
English
Abstract
This study was conducted to evaluate the effect of two disinfection methods (DES1 & DES2) on the establishment of in vitro cultured explants of banana cultivars Tropical and Galil 18. After disinfection treatment, cultures were visually observed daily for contamination.
All contamination was of bacterial origin.
Fifteen colonies from the contaminated cultures were isolated and subjected to tests for hypersensivity reaction (HR), Gram Coloration, and genera identification using the fatty acids test by gas chromatography (Agilant 6890). Significant differences between the two aseptic treatments occurred only for Tropical cv. (X2 = 0.0291). The second aseptic method (DES2) promoted the lowest contamination level, with an average of 7.14% explants contaminated.
None of the 12 isolates under evaluation showed hypersensitivity reaction, and were classified as Gram-negative bacteria.
Seven different genera were identified as Salmonella sp., Pseudomonas huttiensi, Enterobacter cloacae, Enterobacter gergoviae, Paenibacillus polymyxa – Bacillus, Erwinia chrysanthemi biovar.
IV, and Klebsiella pneumoniae pneumoniae.
These results demonstrate the wide diversity of bacterial species found in the banana explant used for initiation, as well as the need to identify a disinfection protocol that can be applied to different banana varieties.
DES2 method worked well with both cultivars but DES1 was significantly less effective in the cultivar Tropical.
All contamination was of bacterial origin.
Fifteen colonies from the contaminated cultures were isolated and subjected to tests for hypersensivity reaction (HR), Gram Coloration, and genera identification using the fatty acids test by gas chromatography (Agilant 6890). Significant differences between the two aseptic treatments occurred only for Tropical cv. (X2 = 0.0291). The second aseptic method (DES2) promoted the lowest contamination level, with an average of 7.14% explants contaminated.
None of the 12 isolates under evaluation showed hypersensitivity reaction, and were classified as Gram-negative bacteria.
Seven different genera were identified as Salmonella sp., Pseudomonas huttiensi, Enterobacter cloacae, Enterobacter gergoviae, Paenibacillus polymyxa – Bacillus, Erwinia chrysanthemi biovar.
IV, and Klebsiella pneumoniae pneumoniae.
These results demonstrate the wide diversity of bacterial species found in the banana explant used for initiation, as well as the need to identify a disinfection protocol that can be applied to different banana varieties.
DES2 method worked well with both cultivars but DES1 was significantly less effective in the cultivar Tropical.
Authors
S.T.S. Ganen, S. Nietsche, M.C.T. Pereira, S.T. Reis, A.A. Xavier, T.M. Santos, T.P. Fernandes
Keywords
Musa spp., tissue culture, bacteria
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