Articles
VARIATION IN PLOIDY AND MORPHOLOGY AMONG ANTHER-DERIVED PAPAYA PLANTS
Article number
829_60
Pages
375 – 381
Language
English
Abstract
Anther-derived papaya plants were evaluated to elucidate their usefulness in breeding and commercial fruit production.
A commercial diploid dwarf cultivar, Wonder blight and 40 anther-derived papaya strains were raised in the same greenhouse.
Data were collected based on their sex, ploidy, and morphological features.
Sex analysis involved DNA extraction from young leaves using the standard CTAB method with minor modifications, co-amplification of papain gene with a sex-specific SCAR (sequence-characterized amplified region) marker and photography of UV trans-illuminated gels on Polaroid film.
Ploidy was determined by digesting leaf samples in Partec HR-A solution (cellulase), staining with Partec HR-B solution, and the relative DNA content of the nuclear suspension was measured using a Partec PA flow cytometer.
Morphological data based on sizes of stems, leaves, flowers, fruits, parthenocarpic ability, and fruit yield were also collected.
The anther-derived papaya strains turned out to be all female, but were variable in ploidy and morphology.
The anther-derived plants were of different ploidy, namely, haploids, diploids, triploids, and tetraploids.
Morphologically, even plants of the same ploidy were variable in height, parthenocarpic ability, fruit size, shape, and yield.
In conclusion, the female papaya plants derived from the male gametophyte originated from microspores.
The haploids and diploids are very useful homozygous breeding lines, while the high yielding triploids and tetraploids have a lot of potential for exploitation in commercial production of seedless fruits.
A commercial diploid dwarf cultivar, Wonder blight and 40 anther-derived papaya strains were raised in the same greenhouse.
Data were collected based on their sex, ploidy, and morphological features.
Sex analysis involved DNA extraction from young leaves using the standard CTAB method with minor modifications, co-amplification of papain gene with a sex-specific SCAR (sequence-characterized amplified region) marker and photography of UV trans-illuminated gels on Polaroid film.
Ploidy was determined by digesting leaf samples in Partec HR-A solution (cellulase), staining with Partec HR-B solution, and the relative DNA content of the nuclear suspension was measured using a Partec PA flow cytometer.
Morphological data based on sizes of stems, leaves, flowers, fruits, parthenocarpic ability, and fruit yield were also collected.
The anther-derived papaya strains turned out to be all female, but were variable in ploidy and morphology.
The anther-derived plants were of different ploidy, namely, haploids, diploids, triploids, and tetraploids.
Morphologically, even plants of the same ploidy were variable in height, parthenocarpic ability, fruit size, shape, and yield.
In conclusion, the female papaya plants derived from the male gametophyte originated from microspores.
The haploids and diploids are very useful homozygous breeding lines, while the high yielding triploids and tetraploids have a lot of potential for exploitation in commercial production of seedless fruits.
Authors
F.K. Rimberia, S. Adaniya, Y. Ishimine, T. Etoh
Keywords
diploids, fruit setting rate, haploids, parthenocarpy, seedless fruits, tetraploids, triploids
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