Articles
IN VITRO MUTAGENESIS FOR SALT TOLERANT RAPESEED (BRASSICA NAPUS L.) USING GAMMA IRRADIATION
Article number
829_52
Pages
337 – 340
Language
English
Abstract
Mutation breeding focuses on individual genes to produce acceptable commercial cultivars and remains a significant method for developing new plant cultivars.
In this study, hypocotyls from five genotypes (Pf7045/91, RGS003, Slm046, Okapi, and Opera) were cultured in callus induction media in order to optimize tissue culture and plant regeneration in rapeseed plant tissues.
Plant regeneration was optimized via organogenesis and somatic embryogenesis using various media additives.
The results showed that the best regeneration was observed in the MS media containing 0.1 mg L-1 NAA, 4 mg L-1 BAP, and 5 mg L-1 silver nitrate.
After this primary optimization, new calli were produced and exposed to Gamma rays at doses of 0, 15, 30, 45, 60, 75, 90, 105, and 120 Gy.
A radiosensitive curve was determined to calculate LD50 dose of gamma radiation.
The irradiated cluster calli were transferred to the best regeneration media and a decrease in regeneration was observed.
The selection of regenerated plants tolerant to salinity was done using media containing salt concentrations of 0, 80, and 160 mM L-1 NaCl.
The response to salt was determined by the extent of visible damage to plantlet growth.
As a result, three plants with higher salt tolerance than the control were obtained and were derived from calli irradiated with 30, 45, 120 Gy.
In this study, hypocotyls from five genotypes (Pf7045/91, RGS003, Slm046, Okapi, and Opera) were cultured in callus induction media in order to optimize tissue culture and plant regeneration in rapeseed plant tissues.
Plant regeneration was optimized via organogenesis and somatic embryogenesis using various media additives.
The results showed that the best regeneration was observed in the MS media containing 0.1 mg L-1 NAA, 4 mg L-1 BAP, and 5 mg L-1 silver nitrate.
After this primary optimization, new calli were produced and exposed to Gamma rays at doses of 0, 15, 30, 45, 60, 75, 90, 105, and 120 Gy.
A radiosensitive curve was determined to calculate LD50 dose of gamma radiation.
The irradiated cluster calli were transferred to the best regeneration media and a decrease in regeneration was observed.
The selection of regenerated plants tolerant to salinity was done using media containing salt concentrations of 0, 80, and 160 mM L-1 NaCl.
The response to salt was determined by the extent of visible damage to plantlet growth.
As a result, three plants with higher salt tolerance than the control were obtained and were derived from calli irradiated with 30, 45, 120 Gy.
Authors
E. Mosleh, A. Mohammadi, M. Omidi, S.J. Rastegari
Keywords
somatic embryogenesis
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